English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  ATP-dependent force generation and membrane scission by ESCRT-III and Vps4

Schöneberg, J., Pavlin, M. R., Yan, S., Righini, M., Lee, I.-H., Carlson, L.-A., et al. (2018). ATP-dependent force generation and membrane scission by ESCRT-III and Vps4. Science, 362(6421), 1423-1428. doi:10.1126/science.aat1839.

Item is

Basic

show hide
Genre: Journal Article

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Schöneberg, Johannes1, 2, 3, Author              
Pavlin, Mark Remec2, 4, Author
Yan, Shannon1, 2, Author
Righini, Maurizio5, Author
Lee, Il-Hyung1, 2, Author
Carlson, Lars-Anders1, 2, Author
Bahrami, Amir Houshang3, Author              
Goldman, Daniel H.2, 5, 6, Author
Ren, Xuefeng1, 2, Author
Hummer, Gerhard3, 7, Author              
Bustamante, Carlos1, 2, 4, 5, 6, 8, 9, Author
Hurley, James H.1, 2, 4, 9, Author
Affiliations:
1Department of Molecular and Cell Biology, University of California, Berkeley, USA, ou_persistent22              
2California Institute for Quantitative Biosciences, University of California, Berkeley, USA, ou_persistent22              
3Department of Theoretical Biophysics, Max Planck Institute of Biophysics, Max Planck Society, Max-von-Laue-Straße 3, 60438 Frankfurt am Main, DE, ou_2068292              
4Graduate Group in Biophysics, University of California, Berkeley, USA, ou_persistent22              
5Department of Chemistry, University of California, Berkeley, USA, ou_persistent22              
6Howard Hughes Medical Institute, University of California, Berkeley, USA, ou_persistent22              
7Institute of Biophysics, Goethe University, Frankfurt / Main, Germany, ou_persistent22              
8Department of Physics, University of California, Berkeley, USA, ou_persistent22              
9Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, USA, ou_persistent22              

Content

show
hide
Free keywords: -
 Abstract: The endosomal sorting complexes required for transport (ESCRTs) catalyze reverse-topology scission from the inner face of membrane necks in HIV budding, multivesicular endosome biogenesis, cytokinesis, and other pathways. We encapsulated ESCRT-III subunits Snf7, Vps24, and Vps2 and the AAA+ ATPase (adenosine triphosphatase) Vps4 in giant vesicles from which membrane nanotubes reflecting the correct topology of scission could be pulled. Upon ATP release by photo-uncaging, this system generated forces within the nanotubes that led to membrane scission in a manner dependent upon Vps4 catalytic activity and Vps4 coupling to the ESCRT-III proteins. Imaging of scission revealed Snf7 and Vps4 puncta within nanotubes whose presence followed ATP release, correlated with force generation and nanotube constriction, and preceded scission. These observations directly verify long-standing predictions that ATP-hydrolyzing assemblies of ESCRT-III and Vps4 sever membranes.

Details

show
hide
Language(s): eng - English
 Dates: 2018-09-172018-02-012018-11-072018-12-21
 Publication Status: Published in print
 Pages: 7
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1126/science.aat1839
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Science
  Other : Science
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Washington, D.C. : American Association for the Advancement of Science
Pages: 6 Volume / Issue: 362 (6421) Sequence Number: - Start / End Page: 1423 - 1428 Identifier: ISSN: 0036-8075
CoNE: https://pure.mpg.de/cone/journals/resource/991042748276600_1