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  ATP-dependent force generation and membrane scission by ESCRT-III and Vps4

Schöneberg, J., Pavlin, M. R., Yan, S., Righini, M., Lee, I.-H., Carlson, L.-A., et al. (2018). ATP-dependent force generation and membrane scission by ESCRT-III and Vps4. Science, 362(6421), 1423-1428. doi:10.1126/science.aat1839.

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Datensatz-Permalink: https://hdl.handle.net/21.11116/0000-0002-D2A0-B Versions-Permalink: https://hdl.handle.net/21.11116/0000-0002-D2A1-A
Genre: Zeitschriftenartikel

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 Urheber:
Schöneberg, Johannes1, 2, 3, Autor           
Pavlin, Mark Remec2, 4, Autor
Yan, Shannon1, 2, Autor
Righini, Maurizio5, Autor
Lee, Il-Hyung1, 2, Autor
Carlson, Lars-Anders1, 2, Autor
Bahrami, Amir Houshang3, Autor           
Goldman, Daniel H.2, 5, 6, Autor
Ren, Xuefeng1, 2, Autor
Hummer, Gerhard3, 7, Autor           
Bustamante, Carlos1, 2, 4, 5, 6, 8, 9, Autor
Hurley, James H.1, 2, 4, 9, Autor
Affiliations:
1Department of Molecular and Cell Biology, University of California, Berkeley, USA, ou_persistent22              
2California Institute for Quantitative Biosciences, University of California, Berkeley, USA, ou_persistent22              
3Department of Theoretical Biophysics, Max Planck Institute of Biophysics, Max Planck Society, Max-von-Laue-Straße 3, 60438 Frankfurt am Main, DE, ou_2068292              
4Graduate Group in Biophysics, University of California, Berkeley, USA, ou_persistent22              
5Department of Chemistry, University of California, Berkeley, USA, ou_persistent22              
6Howard Hughes Medical Institute, University of California, Berkeley, USA, ou_persistent22              
7Institute of Biophysics, Goethe University, Frankfurt / Main, Germany, ou_persistent22              
8Department of Physics, University of California, Berkeley, USA, ou_persistent22              
9Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, USA, ou_persistent22              

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 Zusammenfassung: The endosomal sorting complexes required for transport (ESCRTs) catalyze reverse-topology scission from the inner face of membrane necks in HIV budding, multivesicular endosome biogenesis, cytokinesis, and other pathways. We encapsulated ESCRT-III subunits Snf7, Vps24, and Vps2 and the AAA+ ATPase (adenosine triphosphatase) Vps4 in giant vesicles from which membrane nanotubes reflecting the correct topology of scission could be pulled. Upon ATP release by photo-uncaging, this system generated forces within the nanotubes that led to membrane scission in a manner dependent upon Vps4 catalytic activity and Vps4 coupling to the ESCRT-III proteins. Imaging of scission revealed Snf7 and Vps4 puncta within nanotubes whose presence followed ATP release, correlated with force generation and nanotube constriction, and preceded scission. These observations directly verify long-standing predictions that ATP-hydrolyzing assemblies of ESCRT-III and Vps4 sever membranes.

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Sprache(n): eng - English
 Datum: 2018-09-172018-02-012018-11-072018-12-21
 Publikationsstatus: Erschienen
 Seiten: 7
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: DOI: 10.1126/science.aat1839
 Art des Abschluß: -

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Titel: Science
  Andere : Science
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: Washington, D.C. : American Association for the Advancement of Science
Seiten: 6 Band / Heft: 362 (6421) Artikelnummer: - Start- / Endseite: 1423 - 1428 Identifikator: ISSN: 0036-8075
CoNE: https://pure.mpg.de/cone/journals/resource/991042748276600_1