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  Sex-specific phenotypes of histone H4 point mutants establish dosage compensation as the critical function of H4K16 acetylation in Drosophila

Copur, Ö., Gorchakov, A., Finkl, K., Kuroda, M. I., & Müller, J. (2018). Sex-specific phenotypes of histone H4 point mutants establish dosage compensation as the critical function of H4K16 acetylation in Drosophila. Proceedings of the National Academy of Sciences of the United States of America, 115(52), 13336-13341. doi:10.1073/pnas.1817274115.

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Copur, Ömer1, Author           
Gorchakov, Andrey2, Author
Finkl, Katja1, Author           
Kuroda, Mitzi I.2, Author
Müller, Jürg1, Author           
Affiliations:
1Müller, Jürg / Chromatin Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565161              
2external, ou_persistent22              

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Free keywords: MALE X-CHROMOSOME; H4-K16 ACETYLATION; STRUCTURAL BASIS; CHROMATIN; COMPLEX; MOF; NUCLEOSOME; GENES; SIR3; TRANSCRIPTIONScience & Technology - Other Topics; chromatin; histone modification; H4K16 acetylation; dosage compensation complex; Drosophila;
 Abstract: Acetylation of histone H4 at lysine 16 (H4K16) modulates nucleosome-nucleosome interactions and directly affects nucleosome binding by certain proteins. In Drosophila, H4K16 acetylation by the dosage compensation complex subunit Mof is linked to increased transcription of genes on the single X chromosome in males. Here, we analyzed Drosophila containing different H4K16 mutations or lacking Mof protein. An H4K16A mutation causes embryonic lethality in both sexes, whereas an H4K16R mutation permits females to develop into adults but causes lethality in males. The acetyl-mimic mutation H4K16Q permits both females and males to develop into adults. Complementary analyses reveal that males lacking maternally deposited and zygotically expressed Mof protein arrest development during gastrulation, whereas females of the same genotype develop into adults. Together, this demonstrates the causative role of H4K16 acetylation by Mof for dosage compensation in Drosophila and uncovers a previously unrecognized requirement for this process already during the onset of zygotic gene transcription.

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Language(s): eng - English
 Dates: 2018
 Publication Status: Published in print
 Pages: 6
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 000454302600078
DOI: 10.1073/pnas.1817274115
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Title: Proceedings of the National Academy of Sciences of the United States of America
  Other : Proc. Acad. Sci. USA
  Other : Proc. Acad. Sci. U.S.A.
  Other : Proceedings of the National Academy of Sciences of the USA
  Abbreviation : PNAS
Source Genre: Journal
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Publ. Info: Washington, D.C. : National Academy of Sciences
Pages: - Volume / Issue: 115 (52) Sequence Number: - Start / End Page: 13336 - 13341 Identifier: ISSN: 0027-8424
CoNE: https://pure.mpg.de/cone/journals/resource/954925427230