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  Nanometer-scale Multiplexed Super-Resolution Imaging with an Economic 3D-DNA-PAINT Microscope

Auer, A., Schlichthaerle, T., Woehrstein, J. B., Schueder, F., Strauss, M. T., Grabmayr, H., et al. (2018). Nanometer-scale Multiplexed Super-Resolution Imaging with an Economic 3D-DNA-PAINT Microscope. ChemPhysChem, 19(22), 3024-3034. doi:10.1002/cphc.201800630.

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Genre: Journal Article

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 Creators:
Auer, Alexander1, Author              
Schlichthaerle, Thomas1, Author              
Woehrstein, Johannes B.1, Author              
Schueder, Florian1, Author              
Strauss, Maximilian T.1, Author              
Grabmayr, Heinrich1, Author              
Jungmann, Ralf1, Author              
Affiliations:
1Jungmann, Ralf / Molecular Imaging and Bionanotechnology, Max Planck Institute of Biochemistry, Max Planck Society, ou_2149679              

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Free keywords: DNA; MICROTUBULES; PAINT; LIMITChemistry; Physics; DNA; nanotechnology; photophysics; single-molecule microscopy; super-resolution imaging;
 Abstract: Optical super-resolution microscopy is rapidly changing the way imaging studies in the biological and biomedical sciences are conducted. Due to the unique capability of achieving molecular contrast using fluorescent labels and sub-diffraction resolution down to a few tens of nanometers, super-resolution is developing as an attractive imaging modality. While the increased spatial resolution has already enabled structural studies at unprecedented molecular detail, the wide-spread use of super-resolution approaches as a standard characterization technique in biological laboratories has thus far been prevented by mainly two issues: (1) Intricate sample preparation and image acquisition and (2) costly and complex instrumentation. We here introduce a combination of the recently developed super-resolution technique DNA-PAINT (DNA points accumulation for imaging in nanoscale topography) with an easy-to-replicate, custom-built 3D single-molecule microscope (termed liteTIRF) that is an order of magnitude more economic in cost compared to most commercial systems. We assay the performance of our system using synthetic two- and three-dimensional DNA origami structures and show the applicability to single- and multiplexed cellular imaging.

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Language(s): eng - English
 Dates: 2018
 Publication Status: Published in print
 Pages: 11
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 000450672100009
DOI: 10.1002/cphc.201800630
 Degree: -

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Project name : MolMap
Grant ID : 680241
Funding program : ERC Starting Grant
Funding organization : ERC
Project name : Resolve
Grant ID : 790594
Funding program : ERC Proof of Concept Grant
Funding organization : ERC

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Title: ChemPhysChem
Source Genre: Journal
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Affiliations:
Publ. Info: Weinheim, Germany : Wiley-VCH
Pages: - Volume / Issue: 19 (22) Sequence Number: - Start / End Page: 3024 - 3034 Identifier: ISSN: 1439-4235
CoNE: https://pure.mpg.de/cone/journals/resource/954925409790