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  Activity of the SPCA1 Calcium Pump Couples Sphingomyelin Synthesis to Sorting of Secretory Proteins in the Trans-Golgi Network

Deng, Y., Pakdel, M., Blank, B., Sundberg, E. L., Burd, C. G., & von Blume, J. (2018). Activity of the SPCA1 Calcium Pump Couples Sphingomyelin Synthesis to Sorting of Secretory Proteins in the Trans-Golgi Network. Developmental Cell, 47(4), 464-478.e8. doi:10.1016/j.devcel.2018.10.012.

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Genre: Journal Article

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 Creators:
Deng, Yongqiang1, Author
Pakdel, Mehrshad2, Author              
Blank, Birgit2, Author              
Sundberg, Emma L.1, Author
Burd, Christopher G.1, Author
von Blume, Julia2, Author              
Affiliations:
1external, ou_persistent22              
2von Blume, Julia / Molecular Basis of Protein Trafficking, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565173              

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Free keywords: PANCREATIC ACINAR-CELLS; PLASMA-MEMBRANE; ENDOPLASMIC-RETICULUM; GRANULE BIOGENESIS; CARGO; PATHWAY; DIACYLGLYCEROL; DISTINCT; COMPLEX; MAINTENANCECell Biology; Developmental Biology;
 Abstract: How the principal functions of the Golgi apparatus-protein processing, lipid synthesis, and sorting of macromolecules-are integrated to constitute cargo-specific trafficking pathways originating from the trans-Golgi network (TGN) is unknown. Here, we show that the activity of the Golgi localized SPCA1 calcium pump couples sorting and export of secreted proteins to synthesis of new lipid in the TGN membrane. A secreted Ca2+-binding protein, Cab45, constitutes the core component of a Ca2+-dependent, oligomerization-driven sorting mechanism whereby secreted proteins bound to Cab45 are packaged into a TGN-derived vesicular carrier whose membrane is enriched in sphingomyelin, a lipid implicated in TGN-to-cell surface transport. SPCA1 activity is controlled by the sphingomyelin content of the TGN membrane, such that local sphingomyelin synthesis promotes Ca2+ flux into the lumen of the TGN, which drives secretory protein sorting and export, thereby establishing a protein-and lipid-specific secretion pathway.

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Language(s): eng - English
 Dates: 2018-112018
 Publication Status: Published in print
 Pages: 23
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Degree: -

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Project name : project grant (BL 1186/4-1)
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Funding program : -
Funding organization : Deutsche Forschungsgemeinschaft (DFG)

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Title: Developmental Cell
Source Genre: Journal
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Publ. Info: Cambridge, Mass. : Cell Press
Pages: - Volume / Issue: 47 (4) Sequence Number: - Start / End Page: 464 - 478.e8 Identifier: ISSN: 1534-5807
CoNE: https://pure.mpg.de/cone/journals/resource/111006902714134