English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Template-free 2D particle fusion in localization microscopy

Heydarian, H., Schueder, F., Strauss, M. T., van Werkhoven, B., Fazel, M., Lidke, K. A., et al. (2018). Template-free 2D particle fusion in localization microscopy. Nature methods, 15(10), 781-784. doi:10.1038/s41592-018-0136-6.

Item is

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Heydarian, Hamidreza1, Author
Schueder, Florian2, Author           
Strauss, Maximilian T.2, Author           
van Werkhoven, Ben1, Author
Fazel, Mohamadreza1, Author
Lidke, Keith A.1, Author
Jungmann, Ralf2, Author           
Stallinga, Sjoerd1, Author
Rieger, Bernd1, Author
Affiliations:
1external, ou_persistent22              
2Jungmann, Ralf / Molecular Imaging and Bionanotechnology, Max Planck Institute of Biochemistry, Max Planck Society, ou_2149679              

Content

show
hide
Free keywords: SINGLE-MOLECULE LOCALIZATION; SUPERRESOLUTION MICROSCOPY; CRYOELECTRON MICROSCOPY; RESOLUTIONBiochemistry & Molecular Biology;
 Abstract: Methods that fuse multiple localization microscopy images of a single structure can improve signal-to-noise ratio and resolution, but they generally suffer from template bias or sensitivity to registration errors. We present a template-free particle-fusion approach based on an all-to-all registration that provides robustness against individual misregistrations and underlabeling. We achieved 3.3-nm Fourier ring correlation (FRC) image resolution by fusing 383 DNA origami nanostructures with 80% labeling density, and 5.0-nm resolution for structures with 30% labeling density.

Details

show
hide
Language(s): eng - English
 Dates: 2018
 Publication Status: Issued
 Pages: 6
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 000448820100023
DOI: 10.1038/s41592-018-0136-6
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Nature methods
  Other : Nature methods
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: New York, NY : Nature Pub. Group
Pages: - Volume / Issue: 15 (10) Sequence Number: - Start / End Page: 781 - 784 Identifier: ISSN: 1548-7091
CoNE: https://pure.mpg.de/cone/journals/resource/111088195279556