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  Direct induction of microtubule branching by microtubule nucleation factor SSNA1

Basnet, N., Nedozralova, H., Crevenna, A. H., Bodakuntla, S., Schlichthaerle, T., Taschner, M., et al. (2018). Direct induction of microtubule branching by microtubule nucleation factor SSNA1. Nature Cell Biology, 20(10), 1172-1180. doi:10.1038/s41556-018-0199-8.

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 Creators:
Basnet, Nirakar1, Author              
Nedozralova, Hana1, Author              
Crevenna, Alvaro H.2, Author
Bodakuntla, Satish2, Author
Schlichthaerle, Thomas3, Author              
Taschner, Michael4, Author              
Cardone, Giovanni5, Author              
Janke, Carsten2, Author
Jungmann, Ralf3, Author              
Magiera, Maria M.2, Author
Biertuempfel, Christian6, Author              
Mizuno, Naoko1, Author              
Affiliations:
1Mizuno, Naoko / Cellular and Membrane Trafficking, Max Planck Institute of Biochemistry, Max Planck Society, ou_1688137              
2external, ou_persistent22              
3Jungmann, Ralf / Molecular Imaging and Bionanotechnology, Max Planck Institute of Biochemistry, Max Planck Society, ou_2149679              
4Conti, Elena / Structural Cell Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565144              
5Scientific Service Groups, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565170              
6Biertümpfel, Christian / Molecular Mechanisms of DNA Repair, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565143              

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Free keywords: PARTICLE CRYO-EM; ARP2/3 COMPLEX; GTP HYDROLYSIS; GAMMA-TUBULIN; PROTEINS; MICROSCOPY; SPINDLE; AUGMIN; CENTROSOME; RESOLUTIONCell Biology;
 Abstract: Microtubules are central elements of the eukaryotic cytoskeleton that often function as part of branched networks. Current models for branching include nucleation of new microtubules from severed microtubule seeds or from. gamma-tubulin recruited to the side of a pre-existing microtubule. Here, we found that microtubules can be directly remodelled into branched structures by the microtubule-remodelling factor SSNA1 (also known as NA14 or DIP13). The branching activity of SSNA1 relies on its ability to self-assemble into fibrils in a head-to-tail fashion. SSNA1 fibrils guide protofilaments of a microtubule to split apart to form daughter microtubules. We further found that SSNA1 localizes at axon branching sites and has a key role in neuronal development. SSNA1 mutants that abolish microtubule branching in vitro also fail to promote axon development and branching when overexpressed in neurons. We have, therefore, discovered a mechanism for microtubule branching and implicated its role in neuronal development.

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Language(s): eng - English
 Dates: 2018
 Publication Status: Published in print
 Pages: 12
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 000445656400012
DOI: 10.1038/s41556-018-0199-8
 Degree: -

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Project name : ERC-CoG-724209 to N.M., ERC-StG-680241 to R.J.
Grant ID : -
Funding program : -
Funding organization : European Research Council

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Title: Nature Cell Biology
  Other : 'Nat. Cell Biol.'
Source Genre: Journal
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Publ. Info: London : Macmillan Magazines Ltd.
Pages: - Volume / Issue: 20 (10) Sequence Number: - Start / End Page: 1172 - 1180 Identifier: ISSN: 1465-7392
CoNE: https://pure.mpg.de/cone/journals/resource/954925625310