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  Connectivity of centermost chromatophores in Rhodobacter sphaeroides bacteria

Noble, J. M., Lubieniecki, J., Savitzky, B. H., Plitzko, J., Engelhardt, H., Baumeister, W., et al. (2018). Connectivity of centermost chromatophores in Rhodobacter sphaeroides bacteria. Molecular Microbiology, 109(6), 812-825. doi:10.1111/mmi.14077.

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 Creators:
Noble, Jade M.1, Author
Lubieniecki, Johann2, Author              
Savitzky, Benjamin H.1, Author
Plitzko, Jürgen2, Author              
Engelhardt, Harald2, Author              
Baumeister, Wolfgang2, Author              
Kourkoutis, Lena F.1, Author
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1external, ou_persistent22              
2Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565142              

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Free keywords: PHOTOSYNTHETIC MEMBRANE; RHODOPSEUDOMONAS-SPHAEROIDES; RHODOSPIRILLUM RUBRUM; CRYOELECTRON MICROSCOPY; ELECTRON TOMOGRAPHY; VITREOUS SECTIONS; FINE STRUCTURE; VESICLES; ENERGY; CELLSBiochemistry & Molecular Biology; Microbiology;
 Abstract: The size of whole Rhodobacter sphaeroides prevents 3D visualization of centermost chromatophores in their native environment. This study combines cryo-focused ion beam milling with cryo-electron tomography to probe vesicle architecture both in situ and in 3D. Developing chromatophores are membrane-bound buds that remain in topological continuity with the cytoplasmic membrane and detach into vesicles when mature. Mature chromatophores closest to the cell wall are typically isolated vesicles, whereas centermost chromatophores are either linked to neighboring chromatophores or contain smaller, budding structures. Isolated chromatophores comprised a minority of centermost chromatophores. Connections between vesicles in growing bacteria are through similar to 10 nm-long, similar to 5 nm-wide linkers, and are thus physical rather than functional in terms of converting photons to ATP. In cells in the stationary phase, chromatophores fuse with neighboring vesicles, lose their spherical structure, and greatly increase in volume. The fusion and morphological changes seen in older bacteria are likely a consequence of the aging process, and are not representative of connectivity in healthy R. sphaeroides. Our results suggest that chromatophores can adopt either isolated or connected morphologies within a single bacterium. Revealing the organization of chromatophore vesicles throughout the cell is an important step in understanding the photosynthetic mechanisms in R. sphaeroides.

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Language(s): eng - English
 Dates: 2018
 Publication Status: Published in print
 Pages: 14
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 000447152600007
DOI: 10.1111/mmi.14077
 Degree: -

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Title: Molecular Microbiology
  Other : Mol. Microbiol.
Source Genre: Journal
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Publ. Info: Oxford : Blackwell Science
Pages: - Volume / Issue: 109 (6) Sequence Number: - Start / End Page: 812 - 825 Identifier: ISSN: 0950-382X
CoNE: https://pure.mpg.de/cone/journals/resource/954925574950