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  Site-Specific Labeling of Affimers for DNA-PAINT Microscopy

Schlichthaerle, T., Eklund, A. S., Schueder, F., Strauss, M. T., Tiede, C., Curd, A., et al. (2018). Site-Specific Labeling of Affimers for DNA-PAINT Microscopy. Angewandte Chemie International Edition, 57(34), 11060-11063. doi:10.1002/anie.201804020.

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Genre: Journal Article

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 Creators:
Schlichthaerle, Thomas1, Author              
Eklund, Alexandra S.1, Author              
Schueder, Florian1, Author              
Strauss, Maximilian T.1, Author              
Tiede, Christian2, Author
Curd, Alistair2, Author
Ries, Jonas2, Author
Peckham, Michelle2, Author
Tomlinson, Darren C.2, Author
Jungmann, Ralf1, Author              
Affiliations:
1Jungmann, Ralf / Molecular Imaging and Bionanotechnology, Max Planck Institute of Biochemistry, Max Planck Society, ou_2149679              
2external, ou_persistent22              

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Free keywords: SUPERRESOLUTION MICROSCOPY; EXCHANGE-PAINT; NANOBODIES; PROTEINS; BINDING; MOLECULES; BIOLOGY; CELLSChemistry; Affimers; DNA-PAINT; quantitative microscopy; small binders; super-resolution microscopy;
 Abstract: Optical super-resolution techniques allow fluorescence imaging below the classical diffraction limit of light. From a technology standpoint, recent methods are approaching molecular-scale spatial resolution. However, this remarkable achievement is not easily translated to imaging of cellular components, since current labeling approaches are limited by either large label sizes (antibodies) or the sparse availability of small and efficient binders (nanobodies, aptamers, genetically-encoded tags). In this work, we combined recently developed Affimer reagents with site-specific DNA modification for high-efficiency labeling and imaging using DNA-PAINT. We assayed our approach using an actin Affimer. The small DNA-conjugated affinity binders could provide a solution for efficient multitarget super-resolution imaging in the future.

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Language(s): eng - English
 Dates: 2018-082018
 Publication Status: Published in print
 Pages: 4
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 000442863700048
DOI: 10.1002/anie.201804020
 Degree: -

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Project name : MolMap
Grant ID : 680241
Funding program : ERC Starting Grant
Funding organization : ERC

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Title: Angewandte Chemie International Edition
  Other : Angewandte Chemie, International Edition
  Other : Angew. Chem. Int. Ed.
Source Genre: Journal
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Publ. Info: Weinheim : Wiley-VCH
Pages: - Volume / Issue: 57 (34) Sequence Number: - Start / End Page: 11060 - 11063 Identifier: ISSN: 1433-7851
CoNE: https://pure.mpg.de/cone/journals/resource/1433-7851