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  Vacuole-targeted proteins: ins and outs of subcellular localization studies

Carqueijeiro, I., Sepúlveda, L. J., Mosquera, A., Payne, R., Corbin, C., Papon, N., et al. (2018). Vacuole-targeted proteins: ins and outs of subcellular localization studies. In C. Pereira (Ed.), Plant Vacuolar Trafficking (pp. 33-54). New York City: Humania Press. doi:10.1007/978-1-4939-7856-4_4.

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SOC078.pdf (Publisher version), 999KB
 
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 Creators:
Carqueijeiro, Inês, Author
Sepúlveda, Liuda J., Author
Mosquera, Angela, Author
Payne, Richard, Author
Corbin, Cyrielle, Author
Papon, Nicolas, Author
de Bernonville, Thomas Dugé, Author
Besseau, Sébastien, Author
Lanoue, Arnaud, Author
Glévarec, Gaëlle, Author
Clastre, Marc, Author
St-Pierre, Benoit, Author
Atehortùa, Lucia, Author
Giglioli-Guivarc’h, Nathalie, Author
O'Connor, Sarah E.1, Author              
Oudin, Audrey, Author
Courdavault, and Vincent, Author
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1External Organizations, ou_persistent22              

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 Abstract: Accurate and efficient demonstrations of protein localizations to the vacuole or tonoplast remain strict prerequisites to decipher the role of vacuoles in the whole plant cell biology and notably in defence processes. In this chapter, we describe a reliable procedure of protein subcellular localization study through transient transformations of Catharanthus roseus or onion cells and expression of fusions with fluorescent proteins allowing minimizing artefacts of targeting.

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 Dates: 2018-06-192018
 Publication Status: Published in print
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 Identifiers: Other: SOC078
DOI: 10.1007/978-1-4939-7856-4_4
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Title: Plant Vacuolar Trafficking
Source Genre: Book
 Creator(s):
Pereira, Cláudia , Editor
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Publ. Info: New York City : Humania Press
Pages: 220 Volume / Issue: - Sequence Number: - Start / End Page: 33 - 54 Identifier: ISSN: 1064-3745
ISBN: 978-1-4939-7855-7
DOI: 10.1007/978-1-4939-7856-4

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Title: Methods in Molecular Biology
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Pages: - Volume / Issue: 1789 Sequence Number: - Start / End Page: - Identifier: DOI: 10.1007/978-1-4939-7856-4