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  The phosphoenolpyruvate‐dependent phosphotransferase system of Staphylococcus aureus 1. Amino‐acid sequence of the phosphocarrier protein HPr

Beyreuther, K., Raufuss, H., Schrecker, O., & Hengstenberg, W. (1977). The phosphoenolpyruvate‐dependent phosphotransferase system of Staphylococcus aureus 1. Amino‐acid sequence of the phosphocarrier protein HPr. European Journal of Biochemistry, 75(1), 275-286. doi:10.1111/j.1432-1033.1977.tb11527.x.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0002-D9E5-7 Version Permalink: https://hdl.handle.net/21.11116/0000-000A-3132-6
Genre: Journal Article

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 Creators:
Beyreuther, Konrad, Author
Raufuss , Hildegard, Author
Schrecker, Otto1, Author           
Hengstenberg, Wolfgang1, Author           
Affiliations:
1Max Planck Institute for Medical Research, Max Planck Society, ou_1125545              

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 Abstract:
The primary structure of the histidine‐containing phosphocarrier protein HPr of the phosphoenol‐pyruvate‐dependent phosphotransferase system from Staphylococcus aureus was determined by automated Edman degradation. The complete sequence was deduced from the direct analysis of the protein by automated Edman degradation in a liquid‐phase sequencer of Edman and from the sequence of tryptic, thermolytic and cyanogen bromide peptides as obtained by automated Edman degradation in a solid‐phase sequencer of Laursen. The amino‐acid sequence was found to be Met‐Glu‐Gln‐Asn‐Ser‐Tyr‐Val‐Ile‐Ile‐Asp‐Glu‐Thr‐Gly‐Ile‐His‐Ala‐Arg‐Pro‐Ala‐Thr‐Met‐Leu‐Val‐Gln‐Thr‐Ala‐Ser‐Lys‐Phe‐Asp‐Ser‐Ile‐Asp‐Gln‐Gly‐Gly‐Tyr‐Asp‐Ser‐Met‐Gln‐Leu‐Lys‐Ser‐Leu‐Gly‐Val‐Gly‐Lys‐Asp‐Glu‐Glu‐Ile‐Thr‐Ile‐Tyr‐Ser‐Ala‐Asp‐Lys‐Lys‐Glu‐Gly‐Leu‐Thr‐Lys‐Met‐Ser‐Ile‐Val. The 70 residues correspond to a molecular weight of 7685.

The one histidine involved in the phosphotransfer reaction of this protein was found at position 15 as part of a region of the sequence which has no predictable secondary structure. It is suggested that this protein belongs to the group of male proteins with the active center located on a protrusion rather than a cleft.

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Language(s): eng - English
 Dates: 1976-11-172008-06-281977-05
 Publication Status: Issued
 Pages: 12
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 Rev. Type: Peer
 Identifiers: DOI: 10.1111/j.1432-1033.1977.tb11527.x
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Title: European Journal of Biochemistry
Source Genre: Journal
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Publ. Info: Berlin : Published by Springer-Verlag on behalf of the Federation of European Biochemical Societies
Pages: - Volume / Issue: 75 (1) Sequence Number: - Start / End Page: 275 - 286 Identifier: ISSN: 0014-2956
CoNE: https://pure.mpg.de/cone/journals/resource/111097776606040