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  Interaction of DNA with DNA binding proteins III. Infectivity of protein-complexed phage fd DNA in Escherichia coli spheroplasts

Uhlmann, A., & Geider, K. (1977). Interaction of DNA with DNA binding proteins III. Infectivity of protein-complexed phage fd DNA in Escherichia coli spheroplasts. Biochimica et Biophysica Acta: BBA, 474(4), 639-645. doi:10.1016/0005-2787(77)90083-1.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0002-DA67-5 Version Permalink: https://hdl.handle.net/21.11116/0000-0002-DA68-4
Genre: Journal Article

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BBA_474_1977_639.pdf (Any fulltext), 382KB
 
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 Creators:
Uhlmann, A.1, Author           
Geider, Klaus2, Author           
Affiliations:
1Max Planck Institute for Medical Research, Max Planck Society, ou_1125545              
2Emeritus Group Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497712              

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 Abstract: Complex formation of circular, single-stranded phage fd DNA with Escherichia coli DNA binding protein HD or phage fd gene 5 protein keeps infection of E. coli spheroplasts at the level of free phage DNA, whereas complexes of this DNA with E. coli DNA unwinding protein show a strongly reduced efficiency of transfection. Displacement of the unwinding protein by HD protein or gene 5 protein also maintains the poor adsorption of the complexes to spheroplasts. Free E. coli DNA unwinding protein and residual amounts of this protein bound to the DNA may interfere with the adsorption and the uptake of the phage genome.

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Language(s): eng - English
 Dates: 1976-06-302003-02-051977-02-16
 Publication Status: Issued
 Pages: 7
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1016/0005-2787(77)90083-1
URI: https://www.ncbi.nlm.nih.gov/pubmed/319836
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Title: Biochimica et Biophysica Acta : BBA
  Other : Biochimica et Biophysica Acta (BBA) - Biomembranes
Source Genre: Journal
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Publ. Info: Amsterdam : Elsevier
Pages: - Volume / Issue: 474 (4) Sequence Number: - Start / End Page: 639 - 645 Identifier: Other: 1879-2642
CoNE: https://pure.mpg.de/cone/journals/resource/18792642