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  Fluorescence protein complementation in microscopy: applications beyone detacting bi-molecular interactions

Avilov, S., & Aleksandrova, N. (2019). Fluorescence protein complementation in microscopy: applications beyone detacting bi-molecular interactions. Methods in Applied Fluorescence, 7, 012001. doi:10.1088/2050-6120/aaef01.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0003-6E19-7 Version Permalink: http://hdl.handle.net/21.11116/0000-0004-E151-2
Genre: Journal Article

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 Creators:
Avilov, Sergiy1, Author              
Aleksandrova, Nataliia2, Author
Affiliations:
1Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, 79108 Freiburg, DE, ou_2243640              
2Institut de Genetique et de Biologie Moleculaire et Cellulaire (IGBMC), Illkirch, France, ou_persistent22              

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Free keywords: fluorescent protein, microscopy, imaging superfolder GFP, self-associating fluorescent protein fragments
 Abstract: Conventional fragments of fluorescent proteins used in bimolecular fluorescence complementation technique (BiFC), form light-emitting species only when they are kept in close proximity by interacting proteins of interest. By contrast, certain fluorescent protein fragments complement spontaneously, namely those corresponding to the 1st to 10th beta-strands (GFP1-10) and the 11th beta-strand of superfolder GFP (GFP11). They were designed as folding reporters for high throughput expression and structure biology. Besides, for light microscopy, self-associating fluorescent protein fragments constitute a valuable and sometimes unique tool. The GFP11 tag is very advantageous when a full-length fluorescent protein cannot be fused to a protein of interest, namely for live imaging of certain pathogens. Self-associating GFP fragments enable live labelling of specific synapses, visualization of proteins topology and their exposure to particular subcellular compartments. Present review aims to attract attention of scientific community to these tools and to inspire their further development and applications.

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Language(s): eng - English
 Dates: 2019
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1088/2050-6120/aaef01
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Title: Methods in Applied Fluorescence
Source Genre: Journal
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Pages: - Volume / Issue: 7 Sequence Number: - Start / End Page: 012001 Identifier: -