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  Photo‐ECM: a blue light photoswitchable synthetic extracellular matrix protein for reversible control overcell–matrix adhesion

Ricken, J., Medda, R., & Wegner, S. (2019). Photo‐ECM: a blue light photoswitchable synthetic extracellular matrix protein for reversible control overcell–matrix adhesion. Advanced Biosystems, e-pub(1800302), 1-10. doi:10.1002/adbi.201800302.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0002-EBF5-1 Version Permalink: http://hdl.handle.net/21.11116/0000-0002-EBF6-0
Genre: Journal Article

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AdvBiophys_e-pup_2019_302.pdf (Any fulltext), 835KB
 
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 Creators:
Ricken, Julia1, 2, 3, Author              
Medda, R.2, 4, Author              
Wegner, Seraphine2, 5, Author              
Affiliations:
1Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497699              
2Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              
3Structure of neocortical circuits, Max Planck Institute for Medical Research, Max Planck Society, ou_1497742              
4Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120, Heidelberg, Germany, ou_persistent22              
5Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany, ou_persistent22              

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Free keywords: cell adhesion; extracellular matrix; LOV2; photoswitchable; spatiotemporal control
 Abstract: The dynamic and spatiotemporal control of integrin‐mediated cell adhesion to RGD motifs in its extracellular matrix (ECM) is important for understating cell biology and biomedical applications because cell adhesion fundamentally regulates cellular behavior. Herein, the first photoswitchable synthetic ECM protein, Photo‐ECM, based on the blue light switchable protein LOV2 is engineered. The Photo‐ECM protein includes a RGD sequence, which is hidden in the folded LOV2 protein structure in the dark and is exposed under blue light so that integrins can bind and cells can adhere. The switchable presentation of the RGD motif allows to reversibly mediate and modulate integrin‐based cell adhesions using noninvasive blue light. With this protein cell adhesions in live cells could be reversed and the dynamics at the cellular level is observed. Hence, the Photo‐ECM opens a new possibility to investigate the spatiotemporal regulation of cell adhesions in cell biology and is the first step toward a genetically encoded and light‐responsive ECM.

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Language(s): eng - English
 Dates: 2019-01-142018-11-082019-01-292019-01-29
 Publication Status: Published in print
 Pages: 10
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 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1002/adbi.201800302
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Title: Advanced Biosystems
Source Genre: Journal
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Publ. Info: Weinheim : Wiley-VCH
Pages: - Volume / Issue: e-pub (1800302) Sequence Number: - Start / End Page: 1 - 10 Identifier: ISSN: 2366-7478
CoNE: https://pure.mpg.de/cone/journals/resource/2366-7478