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  Sample delivery for serial crystallography at free-electron lasers and synchrotrons

Grünbein, M. L., & Nass Kovacs, G. (2019). Sample delivery for serial crystallography at free-electron lasers and synchrotrons. Acta Crystallographica Section D: Structural Biology, 75(2), 178-191. doi:10.1107/S205979831801567X.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0002-F2C8-B Version Permalink: http://hdl.handle.net/21.11116/0000-0003-AC7E-F
Genre: Journal Article

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ActaCrystD_75_2019_178.pdf (Any fulltext), 514KB
 
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 Creators:
Grünbein, Marie Luise1, Author              
Nass Kovacs, Gabriela1, Author              
Affiliations:
1Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society, ou_1497700              

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Free keywords: serial sample delivery; high-speed liquid jets; liquid injection; viscous matrices; high-viscosity extrusion; X-ray free-electron lasers; serial crystallography
 Abstract: The high peak brilliance and femtosecond pulse duration of X-ray free-electron lasers (XFELs) provide new scientific opportunities for experiments in physics, chemistry and biology. In structural biology, one of the major applications is serial femtosecond crystallography. The intense XFEL pulse results in the destruction of any exposed microcrystal, making serial data collection mandatory. This requires a high-throughput serial approach to sample delivery. To this end, a number of such sample-delivery techniques have been developed, some of which have been ported to synchrotron sources, where they allow convenient low-dose data collection at room temperature. Here, the current sample-delivery techniques used at XFEL and synchrotron sources are reviewed, with an emphasis on liquid injection and high-viscosity extrusion, including their application for time-resolved experiments. The challenges associated with sample delivery at megahertz repetition-rate XFELs are also outlined.

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Language(s): eng - English
 Dates: 2018-06-222018-11-052019-01-092019
 Publication Status: Published in print
 Pages: 14
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1107/S205979831801567X
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Title: Acta Crystallographica Section D: Structural Biology
  Abbreviation : Acta Cryst. D
Source Genre: Journal
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Publ. Info: Chester, England : International Union of Crystallography
Pages: - Volume / Issue: 75 (2) Sequence Number: - Start / End Page: 178 - 191 Identifier: ISSN: 2059-7983
CoNE: https://pure.mpg.de/cone/journals/resource/2059-7983