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  Correlative cryo super-resolution light and electron microscopy on mammalian cells using fluorescent proteins.

Tuijtel, M. W., Koster, A. J., Jakobs, S., Faas, F. G. A., & Sharp, T. H. (2019). Correlative cryo super-resolution light and electron microscopy on mammalian cells using fluorescent proteins. Scientific Reportsvolume, 9(1): 1369. doi:10.1038/s41598-018-37728-8.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0002-F37D-0 Version Permalink: http://hdl.handle.net/21.11116/0000-0002-F381-9
Genre: Journal Article

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Tuijtel, M. W., Author
Koster, A. J., Author
Jakobs, S.1, Author              
Faas, F. G. A., Author
Sharp, T. H., Author
Affiliations:
1Research Group of Mitochondrial Structure and Dynamics, MPI for biophysical chemistry, Max Planck Society, ou_578566              

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 Abstract: Sample fixation by vitrification is critical for the optimal structural preservation of biomolecules and subsequent high-resolution imaging by cryo-correlative light and electron microscopy (cryoCLEM). There is a large resolution gap between cryo fluorescence microscopy (cryoFLM), ~400-nm, and the sub-nanometre resolution achievable with cryo-electron microscopy (cryoEM), which hinders interpretation of cryoCLEM data. Here, we present a general approach to increase the resolution of cryoFLM using cryo-super-resolution (cryoSR) microscopy that is compatible with successive cryoEM investigation in the same region. We determined imaging parameters to avoid devitrification of the cryosamples without the necessity for cryoprotectants. Next, we examined the applicability of various fluorescent proteins (FPs) for single-molecule localisation cryoSR microscopy and found that all investigated FPs display reversible photoswitchable behaviour, and demonstrated cryoSR on lipid nanotubes labelled with rsEGFP2 and rsFastLime. Finally, we performed SR-cryoCLEM on mammalian cells expressing microtubule-associated protein-2 fused to rsEGFP2 and performed 3D cryo-electron tomography on the localised areas. The method we describe exclusively uses commercially available equipment to achieve a localisation precision of 30-nm. Furthermore, all investigated FPs displayed behaviour compatible with cryoSR microscopy, making this technique broadly available without requiring specialised equipment and will improve the applicability of this emerging technique for cellular and structural biology.

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Language(s): eng - English
 Dates: 2019-02-04
 Publication Status: Published online
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 Rev. Method: Peer
 Identifiers: DOI: 10.1038/s41598-018-37728-8
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Title: Scientific Reportsvolume
Source Genre: Journal
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Pages: 11 Volume / Issue: 9 (1) Sequence Number: 1369 Start / End Page: - Identifier: -