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  Mutagenesis of odorant coreceptor Orco fully disrupts foraging but not oviposition behaviors in the hawkmoth Manduca sexta

Fandino, R. A., Haverkamp, A., Bisch-Knaden, S., Zhang, J., Bucks, S., Nguyen Thi, T. A., et al. (2019). Mutagenesis of odorant coreceptor Orco fully disrupts foraging but not oviposition behaviors in the hawkmoth Manduca sexta. Proceedings of the National Academy of Sciences of the United States of America, 116(31), 15677-15685. doi:10.1073/pnas.1902089116.

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http://dx.doi.org/10.1073/pnas.1902089116 (Publisher version)
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 Creators:
Fandino, Richard A.1, Author           
Haverkamp, Alexander2, 3, Author           
Bisch-Knaden, Sonja2, Author           
Zhang, Jin2, Author           
Bucks, Sascha2, Author           
Nguyen Thi, Tu Anh2, Author           
Werckenthin, Achim, Author
Rybak, Jürgen2, Author           
Stengl, Monika, Author
Knaden, Markus1, Author           
Hansson, Bill S.2, Author           
Grosse-Wilde, Ewald2, Author           
Affiliations:
1Research Group Dr. M. Knaden, Insect Behavior, Department of Neuroethology, Prof. B. S. Hansson, MPI for Chemical Ecology, Max Planck Society, ou_421913              
2Department of Evolutionary Neuroethology, Prof. B. S. Hansson, MPI for Chemical Ecology, Max Planck Society, ou_421894              
3IMPRS on Ecological Interactions, MPI for Chemical Ecology, Max Planck Society, Jena, DE, ou_421900              

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 Abstract: The hawkmoth Manduca sexta and one of its preferred hosts in the North American Southwest, Datura wrightii, share a model
insect–plant relationship based on mutualistic and antagonistic
life-history traits. D. wrightii is the innately preferred nectar source
and oviposition host for M. sexta. Hence, the hawkmoth is an important
pollinator while the M. sexta larvae are specialized herbivores
of the plant. Olfactory detection of plant volatiles plays a
crucial role in the behavior of the hawkmoth. In vivo, the odorant
receptor coreceptor (Orco) is an obligatory component for the function
of odorant receptors (ORs), a major receptor family involved in
insect olfaction. We used CRISPR-Cas9 targeted mutagenesis to
knock out (KO) the MsexOrco gene to test the consequences of a
loss of OR-mediated olfaction in an insect–plant relationship. Neurophysiological
characterization revealed severely reduced antennal
and antennal lobe responses to representative odorants emitted by
D. wrightii. In a wind-tunnel settingwith a flowering plant, Orco KO
hawkmoths showed disrupted flight orientation and an ablated
proboscis extension response to the natural stimulus. The Orco KO
gravid female displayed reduced attraction toward a nonflowering
plant. However, more than half of hawkmoths were able to use
characteristic odor-directed flight orientation and oviposit on the
host plant. Overall, OR-mediated olfaction is essential for foraging
and pollination behaviors, but plant-seeking and oviposition behaviors are sustained through additional OR-independent sensory cues.

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 Dates: 2019-02-052019-07-182019-07-30
 Publication Status: Published in print
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 Rev. Type: -
 Identifiers: Other: HAN321
DOI: 10.1073/pnas.1902089116
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Title: Proceedings of the National Academy of Sciences of the United States of America
  Other : Proc. Acad. Sci. USA
  Other : Proceedings of the National Academy of Sciences of the USA
  Abbreviation : PNAS
Source Genre: Journal
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Publ. Info: Washington, D.C. : National Academy of Sciences
Pages: - Volume / Issue: 116 (31) Sequence Number: - Start / End Page: 15677 - 15685 Identifier: ISSN: 0027-8424
CoNE: https://pure.mpg.de/cone/journals/resource/954925427230