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  Unravelling the zinc finger diversity of recombination regulator PRDM9 in minke whales (Balaenoptera)

Kovalchuk, E. (2018). Unravelling the zinc finger diversity of recombination regulator PRDM9 in minke whales (Balaenoptera). Master Thesis, Christian-Albrechts-Universität zu Kiel, Kiel.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0002-FCDE-9 Version Permalink: http://hdl.handle.net/21.11116/0000-0005-1F6D-0
Genre: Thesis

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 Creators:
Kovalchuk, Elena1, Author              
Roeder, Thomas, Referee
Tautz, Diethard2, Referee              
Odenthal-Hesse, Linda1, Advisor              
Affiliations:
1Research Group Meiotic Recombination and Genome Instability, Department Evolutionary Genetics, Max Planck Institute for Evolutionary Biology, Max Planck Society, ou_2355693              
2Department Evolutionary Genetics, Max Planck Institute for Evolutionary Biology, Max Planck Society, ou_1445635              

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Language(s): eng - English
 Dates: 2018-03-252018-03-25
 Publication Status: Published in print
 Pages: v, 50
 Publishing info: Kiel : Christian-Albrechts-Universität zu Kiel
 Table of Contents: Table of Contents
Affirmation .......................................................................................................................... i
Table of Contents .............................................................................................................. ii
Index of Figures.................................................................................................................iv
Index of Tables ..................................................................................................................iv
Index of Abbreviations ....................................................................................................... v
1 Introduction ................................................................................................................ 1
1.1 PRDM9 .............................................................................................................. 1
1.1.1 PRDM9 in Mus musculus ............................................................................... 2
1.1.2 PRDM9 in Humans ........................................................................................ 4
1.1.3 PRDM9 in non-human Primates ..................................................................... 4
1.1.4 PRDM9 in Ruminants ..................................................................................... 5
1.1.5 PRDM9 in Equidae ......................................................................................... 6
1.2 Balaenoptera Acutorostrata ............................................................................... 7
1.3 Objective .......................................................................................................... 10
2 Materials and Methods ............................................................................................ 11
2.1 B.acutorostrata DNA Samples ......................................................................... 11
2.2 STR genotyping ............................................................................................... 12
2.3 Mitochondrial DLoop Sequencing .................................................................... 13
2.4 PRDM9-ZNF Typing ........................................................................................ 14
3 Results .................................................................................................................... 15
3.1 mtD-Loop Sequencing ..................................................................................... 15
3.2 Microsatellite Analysis ...................................................................................... 17
3.3 PRDM9 Zinc Finger PCR ................................................................................. 19
3.4 PRDM9 Zinc Finger Typing .............................................................................. 20
3.5 Characterising the PRDM9 ZNF Array ............................................................. 21
3.6 In silico C2H2 ZNF binding predictions .............................................................. 22
4 Discussion ............................................................................................................... 24
5 References .............................................................................................................. 31
6 Appendix ................................................................................................................. 37
6.1 Sequences of the used primers........................................................................ 37
 Rev. Method: -
 Identifiers: Other: Dipl/13106
Other: Dipl/13179
 Degree: Master

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