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Language(s):
eng - English
Dates:
2018-03-252018-03-25
Publication Status:
Issued
Pages:
v, 50
Publishing info:
Kiel : Christian-Albrechts-Universität zu Kiel
Table of Contents:
Table of Contents
Affirmation .......................................................................................................................... i
Table of Contents .............................................................................................................. ii
Index of Figures.................................................................................................................iv
Index of Tables ..................................................................................................................iv
Index of Abbreviations ....................................................................................................... v
1 Introduction ................................................................................................................ 1
1.1 PRDM9 .............................................................................................................. 1
1.1.1 PRDM9 in Mus musculus ............................................................................... 2
1.1.2 PRDM9 in Humans ........................................................................................ 4
1.1.3 PRDM9 in non-human Primates ..................................................................... 4
1.1.4 PRDM9 in Ruminants ..................................................................................... 5
1.1.5 PRDM9 in Equidae ......................................................................................... 6
1.2 Balaenoptera Acutorostrata ............................................................................... 7
1.3 Objective .......................................................................................................... 10
2 Materials and Methods ............................................................................................ 11
2.1 B.acutorostrata DNA Samples ......................................................................... 11
2.2 STR genotyping ............................................................................................... 12
2.3 Mitochondrial DLoop Sequencing .................................................................... 13
2.4 PRDM9-ZNF Typing ........................................................................................ 14
3 Results .................................................................................................................... 15
3.1 mtD-Loop Sequencing ..................................................................................... 15
3.2 Microsatellite Analysis ...................................................................................... 17
3.3 PRDM9 Zinc Finger PCR ................................................................................. 19
3.4 PRDM9 Zinc Finger Typing .............................................................................. 20
3.5 Characterising the PRDM9 ZNF Array ............................................................. 21
3.6 In silico C2H2 ZNF binding predictions .............................................................. 22
4 Discussion ............................................................................................................... 24
5 References .............................................................................................................. 31
6 Appendix ................................................................................................................. 37
6.1 Sequences of the used primers........................................................................ 37
Rev. Type:
-
Identifiers:
Other: Dipl/13106
Other: Dipl/13179
Degree:
Master
