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  Tdrd6a Regulates the Aggregation of Buc into Functional Subcellular Compartments that Drive Germ Cell Specification

Roovers, E. F., Kaaij, L. J., Redl, S., Bronkhorst, A. W., Wiebrands, K., de Domingues, A. M. J., et al. (2018). Tdrd6a Regulates the Aggregation of Buc into Functional Subcellular Compartments that Drive Germ Cell Specification. Developmental Cell, 46, 285-301. doi:10.1016/j.devcel.2018.07.009.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0003-6E29-5 Version Permalink: http://hdl.handle.net/21.11116/0000-0004-E630-2
Genre: Journal Article

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 Creators:
Roovers, Elke F.1, Author
Kaaij, Lucas J.T.1, Author
Redl, Stefan1, Author
Bronkhorst, Alfred W.1, Author
Wiebrands, Kay1, Author
de Domingues, António M. Jesus1, Author
Huang, Hsin-Yi1, Author
Han, Chung-Ting1, Author
Riemer, Stephan1, Author
Dosch, Roland1, Author
Salvenmoser, Willi1, Author
Grün, Dominic2, Author              
Butter, Falk1, Author
van Oudenaarden, Alexander1, Author
Ketting, René F.1, Author
Affiliations:
1External Organizations, ou_persistent22              
2Department Independent Research Groups, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243642              

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 Abstract: Phase separation represents an important form of subcellular compartmentalization. However, relatively little is known about how the formation or disassembly of such compartments is regulated. In zebrafish, the Balbiani body (Bb) and the germ plasm (Gp) are intimately linked phase-separated structures essential for germ cell specification and home to many germ cell-specific mRNAs and proteins. Throughout development, these structures occur as a single large aggregate (Bb), which disperses throughout oogenesis and upon fertilization accumulates again into relatively large assemblies (Gp). Formation of the Bb requires Bucky ball (Buc), a protein with prion-like properties. We found that the multi-tudor domain-containing protein Tdrd6a interacts with Buc, affecting its mobility and aggregation properties. Importantly, lack of this regulatory interaction leads to significant defects in germ cell development. Our work presents insights into how prion-like protein aggregations can be regulated and highlights the biological relevance of such regulatory events.

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Language(s): eng - English
 Dates: 2018-08-06
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1016/j.devcel.2018.07.009
 Degree: -

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Title: Developmental Cell
Source Genre: Journal
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Publ. Info: Cambridge, Mass. : Cell Press
Pages: - Volume / Issue: 46 Sequence Number: - Start / End Page: 285 - 301 Identifier: ISSN: 1534-5807
CoNE: https://pure.mpg.de/cone/journals/resource/111006902714134