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  Cryo-Electron Tomography of the Mammalian Synapse

Fernandez-Busnadiego, R. (2018). Cryo-Electron Tomography of the Mammalian Synapse. In L. Swan (Ed.), Clathrin-Mediated Endocytosis (pp. 217-224). New York, NY: Humana Press.

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 Creators:
Fernandez-Busnadiego, Ruben1, Author              
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1Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565142              

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Free keywords: Cryo-electron microscopy Cryo-EM Neuron Tissue fractionation; Synaptic vesicle; Postsynaptic density
 Abstract: Characterizing the detailed structure of the mammalian synapse is of crucial importance to understand its mechanisms of function. Here I describe a protocol to study synaptic architecture by cryo-electron tomography (cryo-ET), a powerful electron microscopy technique that enables 3D visualization of unstained, fully hydrated cellular structures at molecular resolution. The protocol focuses on purified synaptic terminals (“synaptosomes”), currently the most suitable preparation to analyze mammalian synaptic architecture by cryo-ET.

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 Dates: 2018
 Publication Status: Published in print
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Title: Clathrin-Mediated Endocytosis
Source Genre: Book
 Creator(s):
Swan, L., Editor
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Publ. Info: New York, NY : Humana Press
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: 217 - 224 Identifier: ISSN: 1064-3745
ISBN: 978-1-4939-8719-1; 978-1-4939-8717-7

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Title: Methods in Molecular Biology
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Pages: - Volume / Issue: 1847 Sequence Number: - Start / End Page: - Identifier: ISSN: 1064-3745