English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Filter-Aided Sample Preparation for Proteome Analysis.

Wiśniewski, J. R. (2018). Filter-Aided Sample Preparation for Proteome Analysis. In D. Becher (Ed.), Microbial Proteomics. (pp. 3-10). New York, NY: Humana Press.

Item is

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Wiśniewski, Jacek R.1, Author              
Affiliations:
1Mann, Matthias / Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565159              

Content

show
hide
Free keywords: Proteomic sample preparation Sample preparation Proteomic reactor Protein digestion Filter-aided sample preparation (FASP) Multienzyme digestion FASP Bottom-up proteomics
 Abstract: Quantitative protein extraction and high-yield generation of peptides from biological samples are the prerequisite for successful bottom-up type proteomic analysis. Filter aided sample preparation (FASP) is a method for processing of SDS-solubilized cells in a proteomic reactor format. In FASP, disposable centrifugal ultrafiltration units allow for detergent depletion, protein digestion, and isolation of peptides released by proteases from undigested material. Consecutive protein digestion with two or three proteases enables generation of peptide fractions with minimal overlap and considerably increases the number of identifications and protein sequence coverage. FASP is useful for analysis of samples varying in size from a few micrograms to several milligrams of total protein.

Details

show
hide
Language(s):
 Dates: 2018
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 30259475
DOI: 10.1007/978-1-4939-8695-8_1
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Microbial Proteomics.
  Subtitle : Methods in Molecular Biology
Source Genre: Book
 Creator(s):
Becher, D., Editor
Affiliations:
-
Publ. Info: New York, NY : Humana Press
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: 3 - 10 Identifier: -

Source 2

show
hide
Title: Methods in Molecular Biology
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Clifton, N.J. : Humana Press
Pages: - Volume / Issue: 1841 Sequence Number: - Start / End Page: - Identifier: ISSN: 1064-3745
CoNE: https://pure.mpg.de/cone/journals/resource/954927725544