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  Synthesis of Hemicatenanes for the Study of Type IA Topoisomerases.

Lee, S.-H., Hsieh, T.-S., & Siaw, G.-E.-L. (2018). Synthesis of Hemicatenanes for the Study of Type IA Topoisomerases. Methods in Molecular Biology, 1703, 153-159. doi:10.1007/978-1-4939-7459-7_11.

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 Creators:
Lee, Shun-Hsiao1, Author           
Hsieh, Tao-Shih2, Author
Siaw, Grace Ee-Lu2, Author
Affiliations:
1Biertümpfel, Christian / Molecular Mechanisms of DNA Repair, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565143              
2external, ou_persistent22              

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Free keywords: Type IA topoisomerases Hemicatenane Double Holliday junction 2D agarose gel electrophoresis
 Abstract: Hemicatenane is a structure that forms when two DNA duplexes are physically linked through a single-stranded crossover. It is proposed to be an intermediate resulting from double Holliday junction (dHJ) dissolution, repair of replication stalled forks and late stage replication. Our previous study has shown that hemicatenane can be synthesized and dissolved in vitro by hyperthermophilic type IA topoisomerases. Here we present the protocol of hemicatenane synthesis and its structure detection by 2D agarose gel electrophoresis. The generated product can be used as a substrate to study the biochemical mechanism of hemicatenane processing reactions.

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 Dates: 2018
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 29177740
DOI: 10.1007/978-1-4939-7459-7_11
 Degree: -

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Title: Methods in Molecular Biology
  Other : Methods Mol. Biol.
Source Genre: Journal
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Affiliations:
Publ. Info: Clifton, N.J. : Humana Press
Pages: - Volume / Issue: 1703 Sequence Number: - Start / End Page: 153 - 159 Identifier: ISSN: 1064-3745
CoNE: https://pure.mpg.de/cone/journals/resource/954927725544

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Title: DNA Topoisomerases.
Source Genre: Book
 Creator(s):
Drolet, M., Editor
Affiliations:
-
Publ. Info: Humana Press
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: 153 - 159 Identifier: -