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  Mapping the physical network of cellular interactions

Boisset, J.-C., Vivie, J., Grün, D., Muraro, M. J., Lyubimova, A., & van Oudenaarden, A. (2018). Mapping the physical network of cellular interactions. Nature methods, 15, 547-553. doi: 10.1038/s41592-018-0009-z.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0003-6E35-7 Version Permalink: http://hdl.handle.net/21.11116/0000-0004-E634-E
Genre: Journal Article

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 Creators:
Boisset, Jean-Charles1, Author
Vivie, Judith1, Author
Grün, Dominic2, Author              
Muraro, Mauro J.1, Author
Lyubimova, Anna1, Author
van Oudenaarden, Alexander1, Author
Affiliations:
1External Organizations, ou_persistent22              
2Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, 79108 Freiburg, DE, ou_2243640              

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 Abstract: A cell's function is influenced by the environment, or niche, in which it resides. Studies of niches usually require assumptions about the cell types present, which impedes the discovery of new cell types or interactions. Here we describe ProximID, an approach for building a cellular network based on physical cell interaction and single-cell mRNA sequencing, and show that it can be used to discover new preferential cellular interactions without prior knowledge of component cell types. ProximID found specific interactions between megakaryocytes and mature neutrophils and between plasma cells and myeloblasts and/or promyelocytes (precursors of neutrophils) in mouse bone marrow, and it identified a Tac1+ enteroendocrine cell-Lgr5+ stem cell interaction in small intestine crypts. This strategy can be used to discover new niches or preferential interactions in a variety of organs.

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Language(s): eng - English
 Dates: 20182018
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1038/s41592-018-0009-z
 Degree: -

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Title: Nature methods
  Other : Nature methods
Source Genre: Journal
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Publ. Info: New York, NY : Nature Pub. Group
Pages: - Volume / Issue: 15 Sequence Number: - Start / End Page: 547 - 553 Identifier: ISSN: 1548-7091
CoNE: https://pure.mpg.de/cone/journals/resource/111088195279556