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  A protein-binding assay for phallotoxins using muscle actin

Schäfer, A., & Faulstich, H. (1977). A protein-binding assay for phallotoxins using muscle actin. Analytical Biochemistry, 83(2), 720-723. doi:10.1016/0003-2697(77)90077-X.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0002-FFCE-8 Version Permalink: https://hdl.handle.net/21.11116/0000-0002-FFCF-7
Genre: Journal Article

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AnalytBiochem_83_1977_720.pdf (Any fulltext), 228KB
 
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 Creators:
Schäfer, A.1, Author           
Faulstich, Heinz2, Author           
Affiliations:
1Max Planck Institute for Medical Research, Max Planck Society, ou_1125545              
2Department of Molecular Cell Research, Max Planck Institute for Medical Research, Max Planck Society, ou_1497703              

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 Abstract: The high affinity of phallotoxins to filamentous actin (KD = 3.6 × 10−8m) has been used to assay small amounts of phallotoxins. The limit of detection was as low as 160 ng of phallotoxins. This is 50 times lower than the limit of all other procedures known so far. The assay was successfully applied to the analysis of phallotoxins in crude extracts of Amanita pholloides mushrooms.

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Language(s): eng - English
 Dates: 1977-03-301977-08-042004-11-221977-12
 Publication Status: Issued
 Pages: 4
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1016/0003-2697(77)90077-X
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Title: Analytical Biochemistry
Source Genre: Journal
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Publ. Info: San Diego, CA : Academic Press
Pages: - Volume / Issue: 83 (2) Sequence Number: - Start / End Page: 720 - 723 Identifier: ISSN: 0003-2697
CoNE: https://pure.mpg.de/cone/journals/resource/954922644003