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  Beneficial and pathogenic Arabidopsis root-interacting fungi differently affect auxin levels and responsive genes during early infection

Meents, A., Furch, A. C., Almeida-Trapp, M., Özyürek, S., Scholz, S., Kirbis, A., et al. (2019). Beneficial and pathogenic Arabidopsis root-interacting fungi differently affect auxin levels and responsive genes during early infection. Frontiers in Microbiology, 10: 380. doi:10.3389/fmicb.2019.00380.

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http://dx.doi.org/10.3389/fmicb.2019.00380 (Publisher version)
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 Creators:
Meents, Anja1, Author           
Furch, Alexandra C.U., Author
Almeida-Trapp, Marilia2, Author           
Özyürek, Sedef, Author
Scholz, Sandra, Author
Kirbis, Alexander, Author
Lenser, Teresa, Author
Theißen, Günter, Author
Grabe, Veit3, Author           
Hansson, Bill S.4, Author           
Mithöfer, Axel2, Author           
Oelmüller, Ralf, Author
Affiliations:
1IMPRS on Ecological Interactions, MPI for Chemical Ecology, Max Planck Society, Jena, DE, ou_421900              
2Research Group Plant Defense Physiology, Dr. Axel Mithöfer, MPI for Chemical Ecology, Max Planck Society, Jena, DE, ou_3022756              
3Microscopy Service, Dr. Veit Grabe, MPI for Chemical Ecology, Max Planck Society, ou_3171478              
4Department of Evolutionary Neuroethology, Prof. B. S. Hansson, MPI for Chemical Ecology, Max Planck Society, ou_421894              

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 Abstract: Auxin (indole-3-acetic acid, IAA) is an important phytohormone involved in root growth and development. Root-interacting beneficial and pathogenic fungi utilize auxin and its target genes to manipulate the performance of their hosts for their own needs. In order to follow and visualize auxin effects in fungi-colonized Arabidopsis roots, we used the dual auxin reporter construct DR5::EGFP-DR5v2::tdTomato and fluorescence microscopy as well as LC-MS-based phytohormone analyses. We demonstrate that the beneficial endophytic fungi Piriformospora indica and Mortierella hyalina produce and accumulate IAA in their mycelia, in contrast to the phytopathogenic biotrophic fungus Verticillium dahliae and the necrotrophic fungus Alternaria brassicicola. Within three hours after exposure of Arabidopsis roots to the pathogens, the signals of the auxin-responsive reporter genes disappeared. When exposed to P. indica, significantly higher auxin levels and stimulated expression of auxin-responsive reporter genes were detected both in lateral root primordia and the root elongation zone within one day. Elevated auxin levels were also present in the M. hyalina/Arabidopsis root interaction, but no downstream effects on auxin-responsive reporter genes were observed. However, the jasmonate level was strongly increased in the colonized roots. We propose that the lack of stimulated root growth upon infection with M. hyalina is not caused by the absence of auxin, but an inhibitory effect mediated by high jasmonate content.

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 Dates: 2019-02-132019-03-12
 Publication Status: Published online
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 Identifiers: Other: MIT004
DOI: 10.3389/fmicb.2019.00380
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Title: Frontiers in Microbiology
  Abbreviation : Front. Microbiol.
Source Genre: Journal
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Publ. Info: Lausanne : Frontiers Media
Pages: - Volume / Issue: 10 Sequence Number: 380 Start / End Page: - Identifier: ISSN: 1664-302X
CoNE: https://pure.mpg.de/cone/journals/resource/1664-302X