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  H3K9me3-heterochromatin loss at protein-coding genes enables developmental lineage specification

Nicetto, D., Donahue, G., Jain, T., Peng, T., Sidoli, S., Sheng, L., et al. (2019). H3K9me3-heterochromatin loss at protein-coding genes enables developmental lineage specification. Science, 363, 294-297. doi: 10.1126/science.aau0583.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0003-6E4C-E Version Permalink: http://hdl.handle.net/21.11116/0000-0004-DCB5-8
Genre: Journal Article

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 Creators:
Nicetto, Dario1, Author
Donahue, Greg1, Author
Jain, Tanya1, Author
Peng, Tao1, Author
Sidoli, Simone1, Author
Sheng, Lihong1, Author
Montavon, Thomas2, Author
Becker, Justin S.1, Author
Grindheim, Jessica M.1, Author
Blahnik, Kimberly1, Author
Garcia, Benjamin A.1, Author
Tan, Kai1, Author
Bonasio, Roberto1, Author
Jenuwein, Thomas2, Author              
Zaret, Kenneth S.1, Author
Affiliations:
1External Organizations, ou_persistent22              
2Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, 79108 Freiburg, DE, ou_2243640              

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 Abstract: Gene silencing by chromatin compaction is integral to establishing and maintaining cell fates. Trimethylated histone 3 lysine 9 (H3K9me3)-marked heterochromatin is reduced in embryonic stem cells compared to differentiated cells. However, the establishment and dynamics of closed regions of chromatin at protein-coding genes, in embryologic development, remain elusive. We developed an antibody-independent method to isolate and map compacted heterochromatin from low-cell number samples. We discovered high levels of compacted heterochromatin, H3K9me3-decorated, at protein-coding genes in early, uncommitted cells at the germ-layer stage, undergoing profound rearrangements and reduction upon differentiation, concomitant with cell type-specific gene expression. Perturbation of the three H3K9me3-related methyltransferases revealed a pivotal role for H3K9me3 heterochromatin during lineage commitment at the onset of organogenesis and for lineage fidelity maintenance.

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Language(s): eng - English
 Dates: 20192019-01-18
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1126/science.aau0583
 Degree: -

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Title: Science
  Other : Science
Source Genre: Journal
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Publ. Info: Washington, D.C. : American Association for the Advancement of Science
Pages: - Volume / Issue: 363 Sequence Number: - Start / End Page: 294 - 297 Identifier: ISSN: 0036-8075
CoNE: https://pure.mpg.de/cone/journals/resource/991042748276600_1