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  PKC is a target of 7,8,4-trihydroxyisoflavone for the suppression of UVB-induced MMP-1 expression

Lim, T.-G., Kim, Y.-A., Kim, J.-E., Baek, S., Lee, S.-Y., Lee, C. C., et al. (2018). PKC is a target of 7,8,4-trihydroxyisoflavone for the suppression of UVB-induced MMP-1 expression. Experimental Dermatology: an International Journal for Rapid Publication of Short Reports in Experimental Dermatology, 27(5), 449-452. doi:10.1111/exd.13375.

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 Creators:
Lim, Tae-Gyu1, Author
Kim, Yoon-A1, Author
Kim, Jong-Eun1, Author
Baek, Sohee2, Author              
Lee, Sung-Young1, Author
Lee, Charles C.1, Author
Chen, Hanyong1, Author
Kim, Jong Rhan1, Author
Kwon, Jung Yeon1, Author
Bode, Ann M.1, Author
Lee, Chang Yong1, Author
Dong, Zigang1, Author
Oh, Deok-Kun1, Author
Park, Jun Seong1, Author
Lee, Ki Won1, Author
Affiliations:
1external, ou_persistent22              
2Huber, Robert / Structure Research, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565155              

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Free keywords: ULTRAVIOLET-B IRRADIATION; GROWTH-FACTOR RECEPTOR; PROTEIN-KINASE-C; SKIN; ISOFLAVONES; ACTIVATION; CELLSDermatology; 3D culture; 7, 8, 4-trihydroxyisoflavone; matrix metalloproteinase-1; Protein kinase C iota;
 Abstract: The soy isoflavone daidzein is bioconverted to 7,8,4-trihydroxyisoflavone (7,8,4-THIF) by microorganisms. Here, we investigated the matrix metalloproteinase (MMP)-1 inhibitory properties of 7,8,4-THIF that arise through the suppression of UVB-induced MMP-1 expression. 7,8,4-THIF reduced UVB-induced MMP-1 expression at the transcriptional level in primary human dermal fibroblasts and inhibited UVB-induced transcriptional activity of AP-1, a major activator of MMP-1 expression. Additionally, it was observed that the mitogen-activated protein kinase (MAPK) pathway, a crucial signalling cascade for MMP-1 expression, was suppressed by 7,8,4-THIF. Protein kinase C iota (PKC) was suspected to be a direct target of 7,8,4-THIF. The direct interaction between 7,8,4-THIF and PKC was confirmed using pull-down assays and immobilized metal ion affinity-based fluorescence polarization assays. Finally, we observed that 7,8,4-THIF inhibited UVB-induced MMP-1 expression in a human skin equivalent model. Taken together, these results suggest that 7,8,4-THIF, a bioconversion product of daidzein, suppresses UVB-induced MMP-1 expression.

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Language(s): eng - English
 Dates: 2017-042018
 Publication Status: Published in print
 Pages: 4
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 000434355700003
DOI: 10.1111/exd.13375
 Degree: -

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Title: Experimental Dermatology : an International Journal for Rapid Publication of Short Reports in Experimental Dermatology
Source Genre: Journal
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Affiliations:
Publ. Info: Wiley
Pages: - Volume / Issue: 27 (5) Sequence Number: - Start / End Page: 449 - 452 Identifier: ISSN: 0906-6705
CoNE: https://pure.mpg.de/cone/journals/resource/954925562616