English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Determining Neurochemicals from the Non-Human Primate Brain by using Capillary Hydrophilic Interaction Chromatography-Mass Spectrometry

Zhang, X., Rauch, A., Rainer, G., & Logothetis, N. (2009). Determining Neurochemicals from the Non-Human Primate Brain by using Capillary Hydrophilic Interaction Chromatography-Mass Spectrometry. Poster presented at 23rd International Symposium on MicroScale Bioseparations (MSB 2009), Boston, MA, USA.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/21.11116/0000-0003-1805-D Version Permalink: http://hdl.handle.net/21.11116/0000-0003-1807-B
Genre: Poster

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Zhang, X1, 2, Author              
Rauch, A1, 2, Author              
Rainer, G1, 2, Author              
Logothetis, NK1, 2, Author              
Affiliations:
1Department Physiology of Cognitive Processes, Max Planck Institute for Biological Cybernetics, Max Planck Society, ou_1497798              
2Max Planck Institute for Biological Cybernetics, Max Planck Society, ou_1497794              

Content

show
hide
Free keywords: -
 Abstract: Changes in the concentration of neurochemicals, including neurotransmitters, neuromodulators and metabolites, reflect the complex interactions within local and global neural networks. The assessment of such changes promises thus insights into neural communications; in particular if several neurochemicals can be studied at the same time. Here we report findings from chemical analysis of six polar neurochemicals, including acetylcholine (ACh), choline, glutamine, glutamate, lactate and pyruvate, by means of hydrophilic interaction chromatography (HILIC) coupled to tandem mass spectrometry (MS/MS). Extracellular fluid was withdrawn at 40 nl/min by nano push-pull perfusion (NFP3) and collected in vials for sequence analyses. We have successfully obtained and analyzed ECF samples from the primary visual (V1) and extrastriate visual (V4) cortex of awake or anesthetized non-human primates (macaca mulatta). The results showed that all six compounds can be determined by HILIC-MS with low sample treatment requirement. The sensitivity of our measuring system allows the simultaneous monitoring of these six compounds from a 200 nL in vivo sample. We further studied the effects of intracortical application of cholinergic agents in the cortex on the concentration of these chemicals in the extracellular brain fluid. We observed significant increases in extracellular neurochemicals like glutamate and glutamine after the application of ACh or nicotinic agonists. During the ACh application experiments, the profile of exogenously applied ACh and its product choline were monitored, showing that ACh itself remained at low nM range 30 min after injection of 1 mM solution, due to the fast cholinergic metabolism in the brain. All in all, we conclude that capillary HILIC-MS combined with NFP3 is a sensitive technique for simultaneous monitoring of multi-class neurochemicals from extracellular brain samples. Future experiments will concentrate on the effects of the animal's internal state on the concentration of such neurochemicals.

Details

show
hide
Language(s):
 Dates: 2009-02
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: -
 Degree: -

Event

show
hide
Title: 23rd International Symposium on MicroScale Bioseparations (MSB 2009)
Place of Event: Boston, MA, USA
Start-/End Date: 2009-02-01 - 2009-02-05

Legal Case

show

Project information

show

Source 1

show
hide
Title: 23rd International Symposium on MicroScale Bioseparations (MSB 2009)
Source Genre: Proceedings
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: - Sequence Number: P-226-T Start / End Page: 146 - 147 Identifier: -