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  Acute complexin knockout abates spontaneous and evoked transmitter release

López-Murcia, F. J., Reim, K., Jahn, O., Taschenberger, H., & Brose, N. (2019). Acute complexin knockout abates spontaneous and evoked transmitter release. Cell Reports, 26(10), 2521-2530.e5. doi:10.1016/j.celrep.2019.02.030.

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López-Murcia, Francisco José1, Author
Reim, Kerstin1, Author           
Jahn, Olaf2, Author           
Taschenberger, Holger1, Author                 
Brose, Nils1, Author           
Affiliations:
1Molecular neurobiology, Max Planck Institute of Experimental Medicine, Max Planck Society, ou_2173659              
2Proteomics, Wiss. Servicegruppen, Max Planck Institute of Experimental Medicine, Max Planck Society, ou_2173673              

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 Abstract: SNARE-mediated synaptic vesicle (SV) fusion is controlled by multiple regulatory proteins that determine neurotransmitter release efficiency. Complexins are essential SNARE regulators whose mode of action is unclear, as available evidence indicates positive SV fusion facilitation and negative “fusion clamp”-like activities, with the latter occurring only in certain contexts. Because these contradictory findings likely originate in part from different experimental perturbation strategies, we attempted to resolve them by examining a conditional complexin-knockout mouse line as the most stringent genetic perturbation model available. We found that acute complexin loss after synaptogenesis in autaptic and mass-cultured hippocampal neurons reduces SV fusion probability and thus abates the rates of spontaneous, synchronous, asynchronous, and delayed transmitter release but does not affect SV priming or cause “unclamping” of spontaneous SV fusion. Thus, complexins act as facilitators of SV fusion but are dispensable for “fusion clamping” in mammalian forebrain neurons.

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Language(s): eng - English
 Dates: 2019-02-072019-03-05
 Publication Status: Published online
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 Rev. Type: Peer
 Identifiers: DOI: 10.1016/j.celrep.2019.02.030
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Title: Cell Reports
Source Genre: Journal
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Publ. Info: Maryland Heights, MO : Cell Press
Pages: - Volume / Issue: 26 (10) Sequence Number: - Start / End Page: 2521 - 2530.e5 Identifier: ISSN: 2211-1247
CoNE: https://pure.mpg.de/cone/journals/resource/2211-1247