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  Automated laser-assisted synthesis of microarrays for infectious disease research

Paris, G., Heidepriem, J., Tsouka, A., Mende, M., Eickelmann, S., & Löffler, F. F. (2019). Automated laser-assisted synthesis of microarrays for infectious disease research. In B. L. Gray, & H. Becker (Eds.), Microfluidics, BioMEMS, and Medical Microsystems XVII. doi:10.1117/12.2516781.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0003-2471-5 Version Permalink: http://hdl.handle.net/21.11116/0000-0004-C173-0
Genre: Conference Paper

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 Creators:
Paris, Grigori1, Author              
Heidepriem, Jasmin1, Author              
Tsouka, Alexandra1, Author              
Mende, Marco1, Author              
Eickelmann, Stephan1, Author              
Löffler, Felix F.1, Author              
Affiliations:
1Felix Löffler, Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_2385692              

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Free keywords: Laser-induced forward transfer, additive manufacturing, high-density, peptide array, biomarker
 Abstract: We developed a next-generation method for chemical in–situ combinatorial biomolecule array synthesis. This allows for an unprecedented combinatorial freedom in the automated chemical synthesis of molecule arrays with very high spot densities. Key feature of this new method is an automated positioning and laser transfer process: Small solid material spots are rapidly transferred from a donor film to an acceptor surface, requiring only minute amounts of materials. The transfer is performed with different and easy-to-produce donor slides. Each donor slide bears a thin polymer film, embedding one type of monomer. The coupling reaction occurs in a separate heating step, where the matrix becomes viscous and building blocks can diffuse within the material and couple to the acceptor surface. Since these transferred material spots are only several nanometers thin, this method allows for a consecutive multi-layer material deposition of e.g. activation reagents and amino acids. Subsequent heat-induced mixing facilitates an in–situ activation and coupling of the monomers. Positioning several of such resin spots, containing different chemical reagents, on top of each other, will enable for the first time in such small dimensions unique chemical synthesis strategies for each spot. Amount and concentration of the deposited materials can be adjusted with the laser parameters. Employing similar arrays, we can analyze the human immune response towards the proteome of different pathogens. We screened several peptide array replicas with different patient sera. The screenings resulted in significant hits in several proteins with interesting implications for future diagnostics and vaccine development.

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Language(s): eng - English
 Dates: 2019-03-042019
 Publication Status: Published in print
 Pages: -
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 Identifiers: DOI: 10.1117/12.2516781
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Title: SPIE BiOS, 2019
Place of Event: San Francisco, CA, USA
Start-/End Date: 2019-02-02 - 2019-02-07

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Title: Microfluidics, BioMEMS, and Medical Microsystems XVII
Source Genre: Proceedings
 Creator(s):
Gray, Bonnie L., Editor
Becker, Holger, Editor
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Pages: - Volume / Issue: 10875 Sequence Number: 108750C Start / End Page: - Identifier: -