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  An RNA transcribed from DNA at the origin of phage fd single strand to replicative form conversion

Geider, K., Beck, E., & Schaller, H. (1978). An RNA transcribed from DNA at the origin of phage fd single strand to replicative form conversion. Proceedings of the National Academy of Sciences of the United States of America, 75(2), 645-649. doi:10.1073/pnas.75.2.645.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0003-28F5-C Version Permalink: https://hdl.handle.net/21.11116/0000-0003-28F6-B
Genre: Journal Article

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 Creators:
Geider, Klaus1, Author           
Beck, Ewald, Author
Schaller , Heinz, Author
Affiliations:
1Emeritus Group Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497712              

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 Abstract: Phage fd DNA complexed with DNA binding protein I was used by Escherichia coli RNA polymerase (nucleoside triphosphate:RNA nucleotidyltransferase, EC 2.7.7.6) to synthesize an RNA at the origin of single strand to double strand replication. The isolated ori-RNA gave a simple fingerprint after nucleolytic digestion and has a length of about 30 nucleotides. The characterization of the oligonucleotides from the nuclease digest and the extension of the ori-RNA with DNA polymerase I and subsequent restriction of the DNA gave its exact localization in the fd genome, and its total sequence was deduced from the known DNA sequence in this region.

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Language(s): eng - English
 Dates: 1977-10-311978-02-01
 Publication Status: Issued
 Pages: 5
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1073/pnas.75.2.645
URI: https://www.ncbi.nlm.nih.gov/pubmed/204927
URI: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC411312/
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Title: Proceedings of the National Academy of Sciences of the United States of America
  Other : Proc. Acad. Sci. USA
  Other : Proc. Acad. Sci. U.S.A.
  Other : Proceedings of the National Academy of Sciences of the USA
  Abbreviation : PNAS
Source Genre: Journal
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Publ. Info: Washington, D.C. : National Academy of Sciences
Pages: - Volume / Issue: 75 (2) Sequence Number: - Start / End Page: 645 - 649 Identifier: ISSN: 0027-8424
CoNE: https://pure.mpg.de/cone/journals/resource/954925427230