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  High-efficiency generation of fertile transplastomic Arabidopsis plants

Ruf, S., Forner, J., Hasse, C., Kroop, X., Seeger, S., Schollbach, L., et al. (2019). High-efficiency generation of fertile transplastomic Arabidopsis plants. Nature Plants, 5(3), 282-289. doi:10.1038/s41477-019-0359-2.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0003-3121-0 Version Permalink: http://hdl.handle.net/21.11116/0000-0004-4FF0-5
Genre: Journal Article

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 Creators:
Ruf, S.1, Author              
Forner, J.1, Author              
Hasse, C1, Author              
Kroop, X.1, Author              
Seeger, S.1, Author              
Schollbach, L.1, Author              
Schadach, A.1, Author              
Bock, R.1, Author              
Affiliations:
1Organelle Biology and Biotechnology, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753326              

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 Abstract: The development of technologies for the stable genetic transformation of plastid (chloroplast) genomes has been a boon to both basic and applied research. However, extension of the transplastomic technology to major crops and model plants has proven extremely challenging, and the species range of plastid transformation is still very much limited in that most species currently remain recalcitrant to plastid genome engineering. Here, we report an efficient plastid transformation technology for the model plant Arabidopsis thaliana that relies on root-derived microcalli as a source tissue for biolistic transformation. The method produces fertile transplastomic plants at high frequency when combined with a clustered regularly interspaced short palindromic repeats (CRISPR)–CRISPR-associated protein 9 (Cas9)-generated knockout allele of a nuclear locus that enhances sensitivity to the selection agent used for isolation of transplastomic events. Our work makes the model organism of plant biology amenable to routine engineering of the plastid genome, facilitates the combination of plastid engineering with the power of Arabidopsis nuclear genetics, and informs the future development of plastid transformation protocols for other recalcitrant species.

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Language(s): eng - English
 Dates: 2019
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1038/s41477-019-0359-2
Other: Ruf2019
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Title: Nature Plants
Source Genre: Journal
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Publ. Info: Nature Publishing Group
Pages: - Volume / Issue: 5 (3) Sequence Number: - Start / End Page: 282 - 289 Identifier: ISSN: 2055-0278
CoNE: https://pure.mpg.de/cone/journals/resource/2055-0278