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  Fixed-target serial oscillation crystallography at room temperature

Wierman, J. L., Paré-Labrosse, O., Sarracini, A., Besaw, J. E., Cook, M. J., Oghbaey, S., et al. (2019). Fixed-target serial oscillation crystallography at room temperature. IUCrJ, 6, 305-316. doi:10.1107/S2052252519001453.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0003-3387-B Version Permalink: http://hdl.handle.net/21.11116/0000-0003-3388-A
Genre: Journal Article

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https://dx.doi.org/10.1107/S2052252519001453 (Publisher version)
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 Creators:
Wierman, J. L.1, Author
Paré-Labrosse, O.2, 3, Author              
Sarracini, A.2, Author
Besaw, J. E.2, Author
Cook, M. J.1, Author
Oghbaey, S.2, Author
Daoud, H.2, Author
Mehrabi, P.4, Author              
Kriksunov, I.1, Author
Kuo, A.5, Author
Schuller, D. J.1, Author
Smith, S.1, Author
Ernst, O. P.5, Author
Szebenyi, D. M. E.1, Author
Gruner, S. M.1, 6, 7, Author
Miller, R. J. D.2, 4, Author              
Finkea, A. D.1, Author
Affiliations:
1MacCHESS, Cornell University, Ithaca, ou_persistent22              
2Departments of Chemistry and Physics, University of Toronto, ou_persistent22              
3International Max Planck Research School for Ultrafast Imaging & Structural Dynamics (IMPRS-UFAST), Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society, ou_2266714              
4Miller Group, Atomically Resolved Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society, ou_1938288              
5Departments of Biochemistry and Molecular Genetics, University of Toronto, ou_persistent22              
6Department of Physics, Cornell University, Ithaca, ou_persistent22              
7Kavli Institute for Nanoscale Science, Cornell University, Ithaca, ou_persistent22              

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Free keywords: fixed-target serial oscillation; serial crystallography; oscillations; structural biology; radiation damage; structure determination; X-ray crystallography; storage rings
 Abstract: A fixed-target approach to high-throughput room-temperature serial synchrotron crystallography with oscillation is described. Patterned silicon chips with microwells provide high crystal-loading density with an extremely high hit rate. The microfocus, undulator-fed beamline at CHESS, which has compound refractive optics and a fast-framing detector, was built and optimized for this experiment. The high-throughput oscillation method described here collects 1–5° of data per crystal at room temperature with fast (10° s−1) oscillation rates and translation times, giving a crystal-data collection rate of 2.5 Hz. Partial datasets collected by the oscillation method at a storage-ring source provide more complete data per crystal than still images, dramatically lowering the total number of crystals needed for a complete dataset suitable for structure solution and refinement – up to two orders of magnitude fewer being required. Thus, this method is particularly well suited to instances where crystal quantities are low. It is demonstrated, through comparison of first and last oscillation images of two systems, that dose and the effects of radiation damage can be minimized through fast rotation and low angular sweeps for each crystal.

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Language(s): eng - English
 Dates: 2018-11-182019-01-252019-02-23
 Publication Status: Published online
 Pages: 12
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1107/S2052252519001453
 Degree: -

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Title: IUCrJ
Source Genre: Journal
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Publ. Info: Chester CH1 2HU, England : International Union of Crystallography (IUCr)
Pages: 12 Volume / Issue: 6 Sequence Number: - Start / End Page: 305 - 316 Identifier: ISSN: 2052-2525
CoNE: https://pure.mpg.de/cone/journals/resource/2052-2525