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  Mapping developmental maturation of inner hair cell ribbon synapses in the apical mouse cochlea.

Michanski, S., Smaluch, K., Steyer, A. M., Chakrabarti, R., Setz, C., Oestreicher, D., et al. (2019). Mapping developmental maturation of inner hair cell ribbon synapses in the apical mouse cochlea. Proceedings of the National Academy of Sciences of the United States of America, 116(13), 6415-6424. doi:10.1073/pnas.1812029116.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0003-357E-5 Version Permalink: http://hdl.handle.net/21.11116/0000-0003-70D2-1
Genre: Journal Article

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 Creators:
Michanski, S., Author
Smaluch, K., Author
Steyer, A. M., Author
Chakrabarti, R., Author
Setz, C., Author
Oestreicher, D., Author
Fischer, C., Author
Möbius, W., Author
Moser, T.1, Author              
Vogl, C., Author
Wichmann, C., Author
Affiliations:
1Research Group of Synaptic Nanophysiology, MPI for Biophysical Chemistry, Max Planck Society, ou_2205655              

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Free keywords: presynaptic development; ribbon synapse maturation; synaptic heterogeneity; synaptogenesis
 Abstract: Ribbon synapses of cochlear inner hair cells (IHCs) undergo molecular assembly and extensive functional and structural maturation before hearing onset. Here, we characterized the nanostructure of IHC synapses from late prenatal mouse embryo stages (embryonic days 14-18) into adulthood [postnatal day (P)48] using electron microscopy and tomography as well as optical nanoscopy of apical turn organs of Corti. We find that synaptic ribbon precursors arrive at presynaptic active zones (AZs) after afferent contacts have been established. These ribbon precursors contain the proteins RIBEYE and piccolino, tether synaptic vesicles and their delivery likely involves active, microtubule-based transport pathways. Synaptic contacts undergo a maturational transformation from multiple small to one single, large AZ. This maturation is characterized by the fusion of ribbon precursors with membrane-anchored ribbons that also appear to fuse with each other. Such fusion events are most frequently encountered around P12 and hence, coincide with hearing onset in mice. Thus, these events likely underlie the morphological and functional maturation of the AZ. Moreover, the postsynaptic densities appear to undergo a similar refinement alongside presynaptic maturation. Blockwise addition of ribbon material by fusion as found during AZ maturation might represent a general mechanism for modulating ribbon size.

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Language(s): eng - English
 Dates: 2019-03-13
 Publication Status: Published online
 Pages: -
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 Rev. Method: Peer
 Identifiers: DOI: 10.1073/pnas.1812029116
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Title: Proceedings of the National Academy of Sciences of the United States of America
Source Genre: Journal
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Pages: - Volume / Issue: 116 (13) Sequence Number: - Start / End Page: 6415 - 6424 Identifier: -