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  Fine-Tuning Mybl2 is Required for Proper Mesenchymal-to-Epithelial Transition during somatic Reprogramming

Ward, C., Volpe, G., Cauchy, P., Ptasinska, A., Almaghrabi, R., Blakemore, D., et al. (2018). Fine-Tuning Mybl2 is Required for Proper Mesenchymal-to-Epithelial Transition during somatic Reprogramming. Cell Reports, 24, 1496-1511. doi:10.1016/j.celrep.2018.07.026.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0003-6E7C-8 Version Permalink: http://hdl.handle.net/21.11116/0000-0004-E12A-F
Genre: Journal Article

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 Creators:
Ward, Carl1, Author
Volpe, Giacomo1, Author
Cauchy, Pierre2, Author              
Ptasinska, Anetta1, Author
Almaghrabi, Ruba1, Author
Blakemore, Daniel1, Author
Nafria, Monica1, Author
Kestner, Doris1, Author
Frampton, Jon1, Author
Murphy, George1, Author
Buganim, Yosef1, Author
Kaji, Keisuke1, Author
García, Paloma1, Author
Affiliations:
1External Organizations, ou_persistent22              
2Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, 79108 Freiburg, DE, ou_2243640              

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 Abstract: During somatic reprogramming, Yamanaka's pioneer factors regulate a complex sequence of molecular events leading to the activation of a network of pluripotency factors, ultimately resulting in the acquisition and maintenance of a pluripotent state. Here, we show that, contrary to the pluripotency factors studied so far, overexpression of Mybl2 inhibits somatic reprogramming. Our results demonstrate that Mybl2 levels are crucial to the dynamics of the reprogramming process. Mybl2 overexpression changes chromatin conformation, affecting the accessibility of pioneer factors to the chromatin and promoting accessibility for early immediate response genes known to be reprogramming blockers. These changes in the chromatin landscape ultimately lead to a deregulation of key genes that are important for the mesenchymal-to-epithelial transition. This work defines Mybl2 level as a gatekeeper for the initiation of reprogramming, providing further insights into the tight regulation and required coordination of molecular events that are necessary for changes in cell fate identity during the reprogramming process.

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Language(s): eng - English
 Dates: 2018-08-07
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1016/j.celrep.2018.07.026
 Degree: -

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Title: Cell Reports
Source Genre: Journal
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Publ. Info: Maryland Heights, MO : Cell Press
Pages: - Volume / Issue: 24 Sequence Number: - Start / End Page: 1496 - 1511 Identifier: ISSN: 2211-1247
CoNE: https://pure.mpg.de/cone/journals/resource/2211-1247