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  A method for single-stranded ancient DNA library preparation

Gansauge, M.-T., & Meyer, M. (2019). A method for single-stranded ancient DNA library preparation. In B. Shapiro, A. Barlow, P. D. Heintzman, M. Hofreiter, J. L. A. Paijmans, & A. E. R. Soares (Eds.), Ancient DNA: Methods and Protocols (2nd ed., pp. 75-83). New York, NY: Springer.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0003-4B3D-6 Version Permalink: https://hdl.handle.net/21.11116/0000-000C-0926-0
Genre: Book Chapter

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 Creators:
Gansauge, Marie-Theres1, Author                 
Meyer, Matthias1, Author           
Affiliations:
1Advanced DNA Sequencing Techniques, Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Max Planck Society, Deutscher Platz 6, 04103 Leipzig, DE, ou_2074332              

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Free keywords: NGS ; Library preparation ; Ancient DNA ; Single-stranded ligation ; T4 DNA ligase
 Abstract: Genomic library preparation from highly degraded DNA is more efficient when library molecules are prepared separately from the complementary strands of DNA fragments. We describe a protocol in which libraries are constructed from single DNA strands in a three-step procedure: single-stranded ligation of the first adapter with T4 DNA ligase in the presence of a splinter oligonucleotide, copying of the DNA strand with a proofreading polymerase, and blunt-end ligation of the second double-stranded adapter with T4 DNA ligase.

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Language(s): eng - English
 Dates: 2019
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1007/978-1-4939-9176-1_9
 Degree: -

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Title: Ancient DNA: Methods and Protocols
Source Genre: Book
 Creator(s):
Shapiro, Beth, Editor
Barlow, Axel, Editor
Heintzman, Peter D., Editor
Hofreiter, Michael, Editor
Paijmans, Johanna L. A., Editor
Soares, André E. R., Editor
Affiliations:
-
Publ. Info: New York, NY : Springer, 2nd ed.
Pages: x, 216 Volume / Issue: - Sequence Number: - Start / End Page: 75 - 83 Identifier: ISBN: 978-1-4939-9176-1

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Title: Methods in Molecular Biology
Source Genre: Series
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Publ. Info: -
Pages: - Volume / Issue: 1963 Sequence Number: - Start / End Page: - Identifier: -