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  Branched-Chain Amino Acid Catabolism Impacts Triacylglycerol Homeostasis in Chlamydomonas reinhardtii

Liang, Y., Kong, F., Torres-Romero, I., Burlacot, A., Cuine, S., Légeret, B., et al. (2019). Branched-Chain Amino Acid Catabolism Impacts Triacylglycerol Homeostasis in Chlamydomonas reinhardtii. Plant Physiology, 179(4), 1502-1514. doi: 10.1104/pp.18.01584.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0003-5593-7 Version Permalink: http://hdl.handle.net/21.11116/0000-0004-5832-1
Genre: Journal Article

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 Creators:
Liang, Yuanxue1, Author
Kong, Fantao1, Author
Torres-Romero, Ismael1, Author
Burlacot, Adrien1, Author
Cuine, Stéphan1, Author
Légeret, Bertrand1, Author
Billon, Emmanuelle1, Author
Brotman, Y.2, Author              
Alseekh, S.3, Author              
Fernie, A. R.3, Author              
Beisson, Fred1, Author
Peltier, Gilles1, Author
Li-Beisson, Yonghua1, Author
Affiliations:
1external, ou_persistent22              
2Genetics of Metabolic Traits, Cooperative Research Groups, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_2497694              
3Central Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753339              

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 Abstract: Nitrogen (N) starvation-induced triacylglycerol (TAG) synthesis, and its complex relationship with starch metabolism in algal cells, has been intensively studied; however, few studies have examined the interaction between amino acid metabolism and TAG biosynthesis. Here, via a forward genetic screen for TAG homeostasis, we isolated a Chlamydomonas (Chlamydomonas reinhardtii) mutant (bkdE1α) that is deficient in the E1α subunit of the branched-chain ketoacid dehydrogenase (BCKDH) complex. Metabolomics analysis revealed a defect in the catabolism of branched-chain amino acids in bkdE1α. Furthermore, this mutant accumulated 30% less TAG than the parental strain during N starvation and was compromised in TAG remobilization upon N resupply. Intriguingly, the rate of mitochondrial respiration was 20% to 35% lower in bkdE1α compared with the parental strains. Three additional knockout mutants of the other components of the BCKDH complex exhibited phenotypes similar to that of bkdE1α. Transcriptional responses of BCKDH to different N status were consistent with its role in TAG homeostasis. Collectively, these results indicate that branched-chain amino acid catabolism contributes to TAG metabolism by providing carbon precursors and ATP, thus highlighting the complex interplay between distinct subcellular metabolisms for oil storage in green microalgae.

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Language(s): eng - English
 Dates: 2019-04
 Publication Status: Published in print
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Title: Plant Physiology
  Other : Plant Physiol.
Source Genre: Journal
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Publ. Info: Bethesda, Md. : American Society of Plant Biologists
Pages: - Volume / Issue: 179 (4) Sequence Number: - Start / End Page: 1502 - 1514 Identifier: ISSN: 0032-0889
CoNE: https://pure.mpg.de/cone/journals/resource/991042744294438