English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Simple paired heavy- and light-chain antibody repertoire sequencing using endoplasmic reticulum microsomes

Reddy Devullapally, P., Bürger, J., Mielke, T., Konthur, Z., Lehrach, H., Yaspo, M.-L., et al. (2018). Simple paired heavy- and light-chain antibody repertoire sequencing using endoplasmic reticulum microsomes. Genome Medicine, 10: 10:34. doi:10.1186/s13073-018-0542-5.

Item is

Files

show Files
hide Files
:
Devulapally.pdf (Publisher version), 2MB
Name:
Devulapally.pdf
Description:
-
OA-Status:
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
© The Author(s) 2018

Locators

show

Creators

show
hide
 Creators:
Reddy Devullapally, Praneeth1, Author           
Bürger, Jörg2, Author           
Mielke, Thorsten2, Author           
Konthur, Zoltán3, Author           
Lehrach, Hans4, Author           
Yaspo, Marie-Laure1, Author           
Glökler, Jörn, Author
Warnatz, Hans-Jörg1, Author           
Affiliations:
1Gene Regulation and Systems Biology of Cancer (Marie-Laure Yaspo), Independent Junior Research Groups (OWL), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_2117287              
2Microscopy and Cryo-Electron Microscopy (Head: Thorsten Mielke), Scientific Service (Head: Christoph Krukenkamp), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479668              
3Zoltán Konthur, Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863499              
4Emeritus Group of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_2385697              

Content

show
hide
Free keywords: -
 Abstract: Existing methods for paired antibody heavy- and light-chain repertoire sequencing rely on specialized equipment and are limited by their commercial availability and high costs. Here, we report a novel simple and cost-effective emulsion-based single-cell paired antibody repertoire sequencing method that employs only basic laboratory equipment. We performed a proof-of-concept using mixed mouse hybridoma cells and we also showed that our method can be used for discovery of novel antigen-specific monoclonal antibodies by sequencing human CD19+ B cell IgM and IgG repertoires isolated from peripheral whole blood before and seven days after Td (Tetanus toxoid/Diphtheria toxoid) booster immunization. We anticipate broad applicability of our method for providing insights into adaptive immune responses associated with various diseases, vaccinations, and cancer immunotherapies.

Details

show
hide
Language(s): eng - English
 Dates: 2018-04-122018-04-27
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1186/s13073-018-0542-5
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Genome Medicine
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: London, U.K. : BMC
Pages: - Volume / Issue: 10 Sequence Number: 10:34 Start / End Page: - Identifier: ISSN: 1756-994X