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  Simple paired heavy- and light-chain antibody repertoire sequencing using endoplasmic reticulum microsomes

Reddy Devullapally, P., Bürger, J., Mielke, T., Konthur, Z., Lehrach, H., Yaspo, M.-L., et al. (2018). Simple paired heavy- and light-chain antibody repertoire sequencing using endoplasmic reticulum microsomes. Genome Medicine, 10: 10:34. doi:10.1186/s13073-018-0542-5.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0003-55E3-D Version Permalink: http://hdl.handle.net/21.11116/0000-0003-55E4-C
Genre: Journal Article

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 Creators:
Reddy Devullapally, Praneeth1, Author              
Bürger, Jörg2, Author              
Mielke, Thorsten2, Author              
Konthur, Zoltán3, Author              
Lehrach, Hans4, Author              
Yaspo, Marie-Laure1, Author              
Glökler, Jörn, Author
Warnatz, Hans-Jörg1, Author              
Affiliations:
1Gene Regulation and Systems Biology of Cancer (Marie-Laure Yaspo), Independent Junior Research Groups (OWL), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_2117287              
2Microscopy and Cryo-Electron Microscopy (Head: Thorsten Mielke), Scientific Service (Head: Christoph Krukenkamp), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479668              
3Zoltán Konthur, Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863499              
4Emeritus Group of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_2385697              

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 Abstract: Existing methods for paired antibody heavy- and light-chain repertoire sequencing rely on specialized equipment and are limited by their commercial availability and high costs. Here, we report a novel simple and cost-effective emulsion-based single-cell paired antibody repertoire sequencing method that employs only basic laboratory equipment. We performed a proof-of-concept using mixed mouse hybridoma cells and we also showed that our method can be used for discovery of novel antigen-specific monoclonal antibodies by sequencing human CD19+ B cell IgM and IgG repertoires isolated from peripheral whole blood before and seven days after Td (Tetanus toxoid/Diphtheria toxoid) booster immunization. We anticipate broad applicability of our method for providing insights into adaptive immune responses associated with various diseases, vaccinations, and cancer immunotherapies.

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Language(s): eng - English
 Dates: 2018-04-122018-04-27
 Publication Status: Published online
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 Identifiers: DOI: 10.1186/s13073-018-0542-5
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Title: Genome Medicine
Source Genre: Journal
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Publ. Info: London, U.K. : BMC
Pages: - Volume / Issue: 10 Sequence Number: 10:34 Start / End Page: - Identifier: ISSN: 1756-994X