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  Intersectional monosynaptic tracing for dissecting subtype-specific organization of GABAergic interneuron inputs

Yetman, M. J., Washburn, E., Hyun, J. H., Osakada, F., Hayano, Y., Zeng, H., et al. (2019). Intersectional monosynaptic tracing for dissecting subtype-specific organization of GABAergic interneuron inputs. Nature Neuroscience, 22(3), 492-502. doi:10.1038/s41593-018-0322-y.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0003-6894-1 Version Permalink: http://hdl.handle.net/21.11116/0000-0003-6895-0
Genre: Journal Article

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 Creators:
Yetman, Michael J.1, Author
Washburn, Eric1, Author
Hyun, Jung Ho1, Author
Osakada, Fumitaka1, Author
Hayano, Yasufumi1, Author
Zeng, Hongkui1, Author
Callaway, Edward M.1, Author
Kwon, Hyung-Bae2, Author              
Taniguchi, Hiroki1, Author
Affiliations:
1external, ou_persistent22              
2MPI of Neurobiology, Max Planck Society, ou_1110547              

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Free keywords: PRIMARY VISUAL-CORTEX; GENE-EXPRESSION; BARREL CORTEX; CELL SUBTYPES; NEURONS; CONNECTIVITY; CIRCUITS; IDENTIFICATION; INHIBITION; ACCESSNeurosciences & Neurology;
 Abstract: Functionally and anatomically distinct cortical substructures, such as areas or layers, contain different principal neuron (PN) subtypes that generate output signals representing particular information. Various types of cortical inhibitory interneurons (INs) differentially but coordinately regulate PN activity. Despite a potential determinant for functional specialization of PN subtypes, the spatial organization of IN subtypes that innervate defined PN subtypes remains unknown. Here we develop a genetic strategy combining a recombinase-based intersectional labeling method and rabies viral monosynaptic tracing, which enables subtype-specific visualization of cortical IN ensembles sending inputs to defined PN subtypes. Our approach reveals not only cardinal but also underrepresented connections between broad, non-overlapping IN subtypes and PNs. Furthermore, we demonstrate that distinct PN subtypes defined by areal or laminar positions display different organization of input IN subtypes. Our genetic strategy will facilitate understanding of the wiring and developmental principles of cortical inhibitory circuits at unparalleled levels.

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Language(s): eng - English
 Dates: 2019
 Publication Status: Published in print
 Pages: 13
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: ISI: 000459423800020
DOI: 10.1038/s41593-018-0322-y
 Degree: -

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Title: Nature Neuroscience
  Other : Nat. Neurosci.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: New York, NY : Nature America Inc.
Pages: - Volume / Issue: 22 (3) Sequence Number: - Start / End Page: 492 - 502 Identifier: ISSN: 1097-6256
CoNE: https://pure.mpg.de/cone/journals/resource/954925610931