English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  SNAREs define targeting specificity of trafficking vesicles by combinatorial interaction with tethering factors.

Koike, S., & Jahn, R. (2019). SNAREs define targeting specificity of trafficking vesicles by combinatorial interaction with tethering factors. Nature Communicationsvolume, 10: 1608. doi:10.1038/s41467-019-09617-9.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/21.11116/0000-0003-5FA0-E Version Permalink: http://hdl.handle.net/21.11116/0000-0003-5FA6-8
Genre: Journal Article

Files

show Files
hide Files
:
3047325.pdf (Publisher version), 6MB
Name:
3047325.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
:
3047325_Suppl_1.pdf (Supplementary material), 7MB
Name:
3047325_Suppl_1.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
3047325_Suppl_2.pdf (Supplementary material), 185KB
Name:
3047325_Suppl_2.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
3047325_Suppl_3.pdf (Supplementary material), 86KB
Name:
3047325_Suppl_3.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Koike, S.1, Author              
Jahn, R.1, Author              
Affiliations:
1Department of Neurobiology, MPI for Biophysical Chemistry, Max Planck Society, ou_578595              

Content

show
hide
Free keywords: -
 Abstract: Membrane traffic operates by vesicles that bud from precursor organelles and are transported to their target compartment where they dock and fuse. Targeting requires tethering factors recruited by small GTPases and phosphoinositides whereas fusion is carried out by SNARE proteins. Here we report that vesicles containing the Q-SNAREs syntaxin 13 (Stx13) and syntaxin 6 (Stx6) together are targeted to a different endosomal compartment than vesicles containing only Stx6 using injection of artificial vesicles. Targeting by Stx6 requires Vps51, a component of the GARP/EARP tethering complexes. In contrast, targeting by both Stx6 and Stx13 is governed by Vps13B identified here as tethering factor functioning in transport from early endosomes to recycling endosomes. Vps13B specifically binds to Stx13/Stx6 as well as to Rab14, Rab6, and PtdIns(3)P. We conclude that SNAREs use a combinatorial code for recruiting tethering factors, revealing a key function in targeting that is independent of SNARE pairing during fusion.

Details

show
hide
Language(s): eng - English
 Dates: 2019-04-08
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1038/s41467-019-09617-9
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Nature Communicationsvolume
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: 16 Volume / Issue: 10 Sequence Number: 1608 Start / End Page: - Identifier: -