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  Accurate determination of 1H‐15N dipolar couplings using inaccurate settings of the magic angle in solid‐state NMR spectroscopy.

Xue, K., Mühlbauer, M., Mamone, S., Sarkar, R., & Reif, B. (2019). Accurate determination of 1H‐15N dipolar couplings using inaccurate settings of the magic angle in solid‐state NMR spectroscopy. Angewandte Chemie International Edition, 58(13), 4286-4290. doi:10.1002/anie.201814314.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0003-63D3-F Version Permalink: http://hdl.handle.net/21.11116/0000-0003-63D7-B
Genre: Journal Article

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 Creators:
Xue, K., Author
Mühlbauer, M., Author
Mamone, S.1, Author              
Sarkar, R., Author
Reif, B., Author
Affiliations:
1Research Group of NMR Signal Enhancement, MPI for Biophysical Chemistry, Max Planck Society, ou_2396691              

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Free keywords: analytical methods; NMR spectroscopy; proteins; solid-state experiments; structure elucidation
 Abstract: Magic-angle spinning (MAS) is an essential ingredient in a wide variety of solid-state NMR experiments. The standard procedures to adjust the rotor angle are not highly accurate, resulting in a slight misadjustment of the rotor from the magic angle (RL= ) on the order of a few millidegrees. This small missetting has no significant impact on the overall spectral resolution, but is sufficient to reintroduce anisotropic interactions. Shown here is that site-specific H-1-N-15 dipolar couplings can be accurately measured in a heavily deuterated protein. This method can be applied at arbitrarily high MAS frequencies, since neither rotor synchronization nor particularly high radiofrequency field strengths are required. The off-MAS method allows the quantification of order parameters for very dynamic residues, which often escape an analysis using existing methods.

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Language(s): eng - English
 Dates: 2019-01-292019-03-22
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1002/anie.201814314
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Title: Angewandte Chemie International Edition
Source Genre: Journal
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Pages: - Volume / Issue: 58 (13) Sequence Number: - Start / End Page: 4286 - 4290 Identifier: -