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  eDetect: A Fast Error Detection and Correction Tool for Live Cell Imaging Data Analysis

Han, H., Wu, G., Li, Y., & Zi, Z. (2019). eDetect: A Fast Error Detection and Correction Tool for Live Cell Imaging Data Analysis. iScience, 13, 1-8. doi:10.1016/j.isci.2019.02.004.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0003-6692-5 Version Permalink: http://hdl.handle.net/21.11116/0000-0003-6693-4
Genre: Journal Article

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Han.pdf (Publisher version), 3MB
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© 2019 The Author(s)

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 Creators:
Han, Hongqing1, Author              
Wu, Guoyu1, Author              
Li, Yuchao1, Author              
Zi, Zhike1, Author              
Affiliations:
1Cell Signaling Dynamics (Zhike Zi), Independent Junior Research Groups (OWL), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_2117284              

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Free keywords: Automation in Bioinformatics; Bioinformatics; Cell Biology
 Abstract: Live cell imaging has been widely used to generate data for quantitative understanding of cellular dynamics. Various applications have been developed to perform automated imaging data analysis, which often requires tedious manual correction. It remains a challenge to develop an efficient curation method that can analyze massive imaging datasets with high accuracy. Here, we present eDetect, a fast error detection and correction tool that provides a powerful and convenient solution for the curation of live cell imaging analysis results. In eDetect, we propose a gating strategy to distinguish correct and incorrect image analysis results by visualizing image features based on principal component analysis. We demonstrate that this approach can substantially accelerate the data correction process and improve the accuracy of imaging data analysis. eDetect is well documented and designed to be user friendly for non-expert users. It is freely available at https://sites.google.com/view/edetect/ and https://github.com/Zi-Lab/eDetect.

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Language(s): eng - English
 Dates: 2019-03-29
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: DOI: 10.1016/j.isci.2019.02.004
 Degree: -

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Title: iScience
Source Genre: Journal
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Publ. Info: Cell Press
Pages: - Volume / Issue: 13 Sequence Number: - Start / End Page: 1 - 8 Identifier: ISSN: 2589-0042