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  Potassium channel-based optogenetic silencing

Bernal Sierra, Y. A., Rost, B. R., Pofahl, M., Fernandes, A. M., Kopton, R. A., Moser, S., et al. (2018). Potassium channel-based optogenetic silencing. Nature Communications, 9: 4611. doi:10.1038/s41467-018-07038-8.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0003-6F31-A Version Permalink: http://hdl.handle.net/21.11116/0000-0003-6F32-9
Genre: Journal Article

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Bernal Sierra, Yinth Andrea1, Author
Rost, Benjamin R.1, Author
Pofahl, Martin1, Author
Fernandes, António M.2, Author              
Kopton, Ramona A.1, Author
Moser, Sylvain2, Author              
Holtkamp, Dominik1, Author
Masala, Nicola1, Author
Beed, Prateep1, Author
Tukker, John J.1, Author
Oldani, Silvia1, Author
Boenigk, Wolfgang, Author
Kohl, Peter1, Author
Baier, Herwig2, Author              
Schneider-Warme, Franziska1, Author
Hegemann, Peter1, Author
Beck, Heinz1, Author
Seifert, Reinhard1, Author
Schmitz, Dietmar1, Author
Affiliations:
1External Organizations, ou_persistent22              
2Department: Genes-Circuits-Behavior / Baier, MPI of Neurobiology, Max Planck Society, ou_1128545              

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 Abstract: Optogenetics enables manipulation of biological processes with light at high spatio-temporal resolution to control the behavior of cells, networks, or even whole animals. In contrast to the performance of excitatory rhodopsins, the effectiveness of inhibitory optogenetic tools is still insufficient. Here we report a two-component optical silencer system comprising photo-activated adenylyl cyclases (PACs) and the small cyclic nucleotide-gated potassium channel SthK. Activation of this 'PAC-K' silencer by brief pulses of low-intensity blue light causes robust and reversible silencing of cardiomyocyte excitation and neuronal firing. In vivo expression of PAC-K in mouse and zebrafish neurons is well tolerated, where blue light inhibits neuronal activity and blocks motor responses. In combination with red-light absorbing channelrhodopsins, the distinct action spectra of PACs allow independent bimodal control of neuronal activity. PAC-K represents a reliable optogenetic silencer with intrinsic amplification for sustained potassium-mediated hyperpolarization, conferring high operational light sensitivity to the cells of interest.

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 Dates: 2018-11-05
 Publication Status: Published online
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Title: Nature Communications
  Abbreviation : Nat. Commun.
Source Genre: Journal
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Publ. Info: London : Nature Publishing Group
Pages: - Volume / Issue: 9 Sequence Number: 4611 Start / End Page: - Identifier: ISSN: 2041-1723
CoNE: https://pure.mpg.de/cone/journals/resource/2041-1723