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  A carbene-generating photoaffinity probe for beta-adrenergic receptors

Burgermeister, W., Nassal, M., Wieland, T., & Helmreich, E. J. M. (1983). A carbene-generating photoaffinity probe for beta-adrenergic receptors. Biochimica et Biophysica Acta-Biomembranes, 729(2), 219-228. doi:10.1016/0005-2736(83)90488-1.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0003-6FAB-1 Version Permalink: http://hdl.handle.net/21.11116/0000-0003-6FAC-0
Genre: Journal Article

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BBA_729_1983_219.pdf (Any fulltext), 815KB
 
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 Creators:
Burgermeister, Wolfgang, Author
Nassal, Michael1, Author              
Wieland, Theodor1, Author              
Helmreich , Ernst J. M., Author
Affiliations:
1Max Planck Institute for Medical Research, Max Planck Society, ou_1125545              

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Free keywords: Photoaffinity probe; Carbene formation; β-Adrenergic receptor; (Turkey erythrocyte, Guinea pig lung)
 Abstract: A new radioiodinated (2.2 Ci/mumol) iodocyanopindolol derivative carrying a 4-(3-trifluoromethyldiazirino)benzoyl residue has been synthesized. The long-wavelength absorption of the diazirine permits formation of the carbene by photolysis under very mild conditions. [125I]ICYP-diazirine binds with high affinity (Kd = 60 pM) to beta-receptors from turkey erythrocyte membranes. Upon irradiation, [125I]ICYP-diazirine is covalently incorporated in a Mr 40 000 protein. Stereoselective inhibition of photolabeling by the (-)enantiomers of alprenolol and isoproterenol indicated that the Mr 40 000 protein contains a beta-adrenergic binding site. The yield of specific labeling was up to 8.2% of total beta-receptor binding sites. The Mr 40 000 protein photolabeled in the membrane could be solubilized at comparable yield with either digitonin or Triton X-100. Irradiation of digitonin-solubilized turkey erythrocyte membranes with [125I]ICYP-diazirine resulted in specific labeling of two proteins with Mr 40 000 and 50 000. In guinea-pig lung membranes, at least five proteins were photolabeled, of which one (with approximately Mr 67 000) was labeled specifically.

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Language(s): eng - English
 Dates: 1982-12-072003-01-281983-04-06
 Publication Status: Published in print
 Pages: 10
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

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Title: Biochimica et Biophysica Acta-Biomembranes
Source Genre: Journal
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Publ. Info: Amsterdam : Elsevier
Pages: - Volume / Issue: 729 (2) Sequence Number: - Start / End Page: 219 - 228 Identifier: ISSN: 0005-2736
CoNE: https://pure.mpg.de/cone/journals/resource/954926938702