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  Ribosome provisioning activates a bistable switch coupled to fast exit from stationary phase

Remigi, P., Rainey, P. B., Ferguson, G. C., Rogers, D. W., McConnell, E., & De Monte, S. (2019). Ribosome provisioning activates a bistable switch coupled to fast exit from stationary phase. Molecular Biology and Evolution, 36(5), 1056-1070. doi:10.1093/molbev/msz041.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0003-70B3-4 Version Permalink: http://hdl.handle.net/21.11116/0000-0004-48DE-2
Genre: Journal Article

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 Creators:
Remigi, Philippe, Author
Rainey, Paul B.1, Author              
Ferguson, Gayle C., Author
Rogers, David W.1, Author              
McConnell, Ellen1, Author              
De Monte, Silvia2, Author              
Affiliations:
1Department Microbial Population Biology, Max Planck Institute for Evolutionary Biology, Max Planck Society, ou_2421699              
2Department Evolutionary Theory, Max Planck Institute for Evolutionary Biology, Max Planck Society, ou_1445641              

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 Abstract: Observations of bacteria at the single-cell level have revealed many instances of phenotypic heterogeneity within otherwise clonal populations, but the selective causes, molecular bases, and broader ecological relevance remain poorly understood. In an earlier experiment in which the bacterium Pseudomonas fluorescens SBW25 was propagated under a selective regime that mimicked the host immune response, a genotype evolved that stochastically switched between capsulation states. The genetic cause was a mutation in carB that decreased the pyrimidine pool (and growth rate), lowering the activation threshold of a preexisting but hitherto unrecognized phenotypic switch. Genetic components surrounding bifurcation of UTP flux toward DNA/RNA or UDP-glucose (a precursor of colanic acid forming the capsules) were implicated as key components. Extending these molecular analyses—and based on a combination of genetics, transcriptomics, biochemistry, and mathematical modeling—we show that pyrimidine limitation triggers an increase in ribosome biosynthesis and that switching is caused by competition between ribosomes and CsrA/RsmA proteins for the mRNA transcript of a positively autoregulated activator of colanic acid biosynthesis. We additionally show that in the ancestral bacterium the switch is part of a program that determines stochastic entry into a semiquiescent capsulated state, ensures that such cells are provisioned with excess ribosomes, and enables provisioned cells to exit rapidly from stationary phase under permissive conditions.

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Language(s): eng - English
 Dates: 2019-03-052019-05
 Publication Status: Published in print
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 Identifiers: DOI: 10.1093/molbev/msz041
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Title: Molecular Biology and Evolution
  Other : Mol. Biol. Evol.
Source Genre: Journal
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Publ. Info: Oxford : Oxford University Press
Pages: - Volume / Issue: 36 (5) Sequence Number: - Start / End Page: 1056 - 1070 Identifier: ISSN: 0737-4038
CoNE: https://pure.mpg.de/cone/journals/resource/954925536119