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  Programmable functionalization of surfactant‐stabilized microfluidic droplets via DNA‐tags

Jahnke, K., Weiss, M., Frey, C., Antona, S., Janiesch, J.-W., Platzman, I., et al. (2019). Programmable functionalization of surfactant‐stabilized microfluidic droplets via DNA‐tags. Advanced Functional Materials, (1808647): e-pup, pp. 1-8. doi:10.1002/adfm.201808647.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0003-736E-1 Version Permalink: http://hdl.handle.net/21.11116/0000-0003-898C-5
Genre: Journal Article

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 Creators:
Jahnke, Kevin1, Author              
Weiss, Marian1, 2, Author              
Frey, Christoph1, 2, Author              
Antona, Silvia1, Author              
Janiesch, Jan-Willi1, 2, Author              
Platzman, Ilia1, 2, Author              
Göpfrich, Kerstin1, Author              
Spatz, Joachim P.1, 2, Author              
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              
2Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany, ou_persistent22              

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Free keywords: block copolymer surfactant; cholesterol‐tagged DNA; DNA nanotechnology; droplet‐based microfluidics; functionalization
 Abstract: Droplet‐based microfluidics has emerged as a powerful tool in synthetic biology. For many applications, chemical functionalization of the droplets is a key process. Therefore, a straightforward and broadly applicable approach is developed to functionalize the inner periphery of microfluidic droplets with diverse reactive groups and components. Instead of covalent modification of the droplet‐stabilizing surfactants, this method relies on cholesterol‐tagged DNA that self‐assembles at the droplet periphery. The cholesterol‐tagged DNA serves as an attachment handle for the recruitment of complementary DNA. The complementary DNA can carry diverse functional groups. We exemplify our method by demonstrating the attachment of amine groups, DNA nanostructures, microspheres, a minimal actin cortex, and leukemia cells to the droplet periphery. It is further shown that the DNA‐mediated attachment to the droplet periphery is temperature‐responsive and reversible. It is envisioned that droplet functionalization via DNA handles will help to tailor droplet interfaces for diverse applications—featuring programmable assembly, unique addressability, and stimuli‐responsiveness.

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Language(s): eng - English
 Dates: 2018-12-052019-03-092019-04-08
 Publication Status: Published in print
 Pages: 8
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1002/adfm.201808647
 Degree: -

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Title: Advanced Functional Materials
  Other : Adv. Funct. Mater.
Source Genre: Journal
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Publ. Info: Weinheim : Wiley-VCH Verlag GmbH
Pages: - Volume / Issue: (1808647) Sequence Number: e-pup Start / End Page: 1 - 8 Identifier: ISSN: 1616-301X
CoNE: https://pure.mpg.de/cone/journals/resource/954925596563