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  Different forms of African cassava mosaic virus capsid protein within plants and virions

Hipp, K., Zikeli, K., Kepp, G., Schmid, L., Shoeman, R. L., P.Jurkowski, T., et al. (2019). Different forms of African cassava mosaic virus capsid protein within plants and virions. Virology, 529, 81-90. doi:10.1016/j.virol.2019.01.018.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0003-7624-0 Version Permalink: http://hdl.handle.net/21.11116/0000-0003-7625-F
Genre: Journal Article

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 Creators:
Hipp, Katharina, Author
Zikeli, Kerstin, Author
Kepp, Gabi, Author
Schmid, Lena, Author
Shoeman, Robert L.1, 2, 3, Author              
P.Jurkowski, Tomasz, Author
Kleinow, Tatjana, Author
Jeske, Holger, Author
Affiliations:
1Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society, ou_1497700              
2Coherent diffractive imaging, Max Planck Institute for Medical Research, Max Planck Society, ou_1497692              
3Analytical Protein Biochemistry, Max Planck Institute for Medical Research, Max Planck Society, ou_1497690              

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Free keywords: Geminivirus; Capsid protein; CP; Virus particles; Mass spectrometry; Ubiquitination
 Abstract: One geminiviral gene encodes the capsid protein (CP), which can appear as several bands after electrophoresis depending on virus and plant. African cassava mosaic virus-Nigeria CP in Nicotiana benthamiana, however, yielded one band (~ 30 kDa) in total protein extracts and purified virions, although its expression in yeast yielded two bands (~ 30, 32 kDa). Mass spectrometry of the complete protein and its tryptic fragments from virions is consistent with a cleaved start M1, acetylated S2, and partial phosphorylation at T12, S25 and S62. Mutants for additional potentially modified sites (N223A; C235A) were fully infectious and formed geminiparticles. Separation in triton acetic acid urea gels confirmed charge changes of the CP between plants and yeast indicating differential phosphorylation. If the CP gene alone was expressed in plants, multiple bands were observed like in yeast. A high turnover rate indicates that post-translational modifications promote CP decay probably via the ubiquitin-triggered proteasomal pathway.

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Language(s): eng - English
 Dates: 2019-01-142018-11-272019-01-162019-01-172019-03
 Publication Status: Published in print
 Pages: 10
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Degree: -

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Title: Virology
Source Genre: Journal
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Publ. Info: Orlando, Fla. : Academic Press
Pages: - Volume / Issue: 529 Sequence Number: - Start / End Page: 81 - 90 Identifier: ISSN: 0042-6822
CoNE: https://pure.mpg.de/cone/journals/resource/954922647063