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  Different forms of African cassava mosaic virus capsid protein within plants and virions

Hipp, K., Zikeli, K., Kepp, G., Schmid, L., Shoeman, R. L., P.Jurkowski, T., et al. (2019). Different forms of African cassava mosaic virus capsid protein within plants and virions. Virology, 529, 81-90. doi:10.1016/j.virol.2019.01.018.

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 Urheber:
Hipp, Katharina, Autor
Zikeli, Kerstin, Autor
Kepp, Gabi, Autor
Schmid, Lena, Autor
Shoeman, Robert L.1, 2, 3, Autor           
P.Jurkowski, Tomasz, Autor
Kleinow, Tatjana, Autor
Jeske, Holger, Autor
Affiliations:
1Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society, ou_1497700              
2Coherent diffractive imaging, Max Planck Institute for Medical Research, Max Planck Society, ou_1497692              
3Analytical Protein Biochemistry, Max Planck Institute for Medical Research, Max Planck Society, ou_1497690              

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Schlagwörter: Geminivirus; Capsid protein; CP; Virus particles; Mass spectrometry; Ubiquitination
 Zusammenfassung: One geminiviral gene encodes the capsid protein (CP), which can appear as several bands after electrophoresis depending on virus and plant. African cassava mosaic virus-Nigeria CP in Nicotiana benthamiana, however, yielded one band (~ 30 kDa) in total protein extracts and purified virions, although its expression in yeast yielded two bands (~ 30, 32 kDa). Mass spectrometry of the complete protein and its tryptic fragments from virions is consistent with a cleaved start M1, acetylated S2, and partial phosphorylation at T12, S25 and S62. Mutants for additional potentially modified sites (N223A; C235A) were fully infectious and formed geminiparticles. Separation in triton acetic acid urea gels confirmed charge changes of the CP between plants and yeast indicating differential phosphorylation. If the CP gene alone was expressed in plants, multiple bands were observed like in yeast. A high turnover rate indicates that post-translational modifications promote CP decay probably via the ubiquitin-triggered proteasomal pathway.

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Sprache(n): eng - English
 Datum: 2019-01-142018-11-272019-01-162019-01-172019-03
 Publikationsstatus: Erschienen
 Seiten: 10
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
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Titel: Virology
Genre der Quelle: Zeitschrift
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Ort, Verlag, Ausgabe: Orlando, Fla. : Academic Press
Seiten: - Band / Heft: 529 Artikelnummer: - Start- / Endseite: 81 - 90 Identifikator: ISSN: 0042-6822
CoNE: https://pure.mpg.de/cone/journals/resource/954922647063