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  Adhesion Stabilized en Masse Intracellular Electrical Recordings from Multicellular Assemblies

Staufer, O., Weber, S., Bengtson, C. P., Bading, H., Rustom, A., & Spatz, J. P. (2019). Adhesion Stabilized en Masse Intracellular Electrical Recordings from Multicellular Assemblies. Nano Letters, e-pup: e-pup, pp. A-L. doi:10.1021/acs.nanolett.9b00784.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0003-7E5F-7 Version Permalink: http://hdl.handle.net/21.11116/0000-0003-7E60-4
Genre: Journal Article

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 Creators:
Staufer, Oskar1, 2, Author              
Weber, Sebastian1, 3, Author              
Bengtson, C. Peter, Author
Bading, Hilmar, Author
Rustom, Amin, Author
Spatz, Joachim P.1, 2, Author              
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              
2Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany, ou_persistent22              
3Biophysikalische Chemie, Universtität Heidelberg, ou_persistent22              

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 Abstract: Coordinated collective electrochemical signals in multicellular assemblies, such as ion fluxes, membrane potentials, electrical gradients, and steady electric fields, play an important role in cell and tissue spatial organization during many physiological processes like wound healing, inflammatory responses, and hormone release. This mass of electric actions cumulates in an en masse activity within cell collectives which cannot be deduced from considerations at the individual cell level. However, continuously sampling en masse collective electrochemical actions of the global electrochemical activity of large-scale electrically coupled cellular assemblies with intracellular resolution over long time periods has been impeded by a lack of appropriate recording techniques. Here we present a bioelectrical interface consisting of low impedance vertical gold nanoelectrode interfaces able to penetrate the cellular membrane in the course of cellular adhesion, thereby allowing en masse recordings of intracellular electrochemical potentials that transverse electrically coupled NRK fibroblast, C2C12 myotube assemblies, and SH-SY5Y neuronal networks of more than 200,000 cells. We found that the intracellular electrical access of the nanoelectrodes correlates with substrate adhesion dynamics and that penetration, stabilization, and sealing of the electrode–cell interface involves recruitment of surrounding focal adhesion complexes and the anchoring of actin bundles, which form a caulking at the electrode base. Intracellular recordings were stable for several days, and monitoring of both basal activity as well as pharmacologically altered electric signals with high signal-to-noise ratios and excellent electrode coupling was performed.

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Language(s): eng - English
 Dates: 2019-04-042019-02-222019-04-05
 Publication Status: Published in print
 Pages: 12
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1021/acs.nanolett.9b00784
 Degree: -

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Title: Nano Letters
  Abbreviation : Nano Lett.
Source Genre: Journal
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Publ. Info: Washington, DC : American Chemical Society
Pages: - Volume / Issue: e-pup Sequence Number: e-pup Start / End Page: A - L Identifier: ISSN: 1530-6984
CoNE: https://pure.mpg.de/cone/journals/resource/110978984570403